• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Comparison of Diagnostic Accuracy of PCR Targeting the 47-Kilodalton Protein Membrane Gene of Treponema pallidum and PCR Targeting the DNA Polymerase I Gene: Systematic Review and Meta-analysis
【24h】

Comparison of Diagnostic Accuracy of PCR Targeting the 47-Kilodalton Protein Membrane Gene of Treponema pallidum and PCR Targeting the DNA Polymerase I Gene: Systematic Review and Meta-analysis

机译：以梅毒螺旋体为靶标的47-千达蛋白膜基因PCR和以DNA聚合酶I基因为靶PCR的诊断准确性比较：系统评价和荟萃分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Treponema pallidum PCR (Tp-PCR) testing now is recommended as a valid tool for the diagnosis of primary or secondary syphilis. The objectives were to systematically review and determine the optimal specific target gene to be used for Tp-PCR. Comparisons of the performance of the two main targets are tpp47 and polA genes were done using meta-analysis. Three electronic bibliographic databases, representing abstract books from five conferences specialized in infectious diseases from January 1990 to March 2015, were searched. Search keywords included (“syphilis” OR “Treponema pallidum” OR “neurosyphilis”) AND (“PCR” OR “PCR” OR “molecular amplification”). We included diagnostic studies assessing the performance of Tp-PCR targeting tpp47 (tpp47-Tp-PCR) or the polA gene (polA-Tp-PCR) in ulcers from early syphilis. All studies were assessed against quality criteria using the QUADAS-2 tool. Of 37 studies identified, 62.2% were judged at low risk of bias or applicability. Most used the U.S. Centers for Disease Control and Prevention (CDC) case definitions for primary or secondary (early) syphilis (89.2%; n = 33); 15 (40.5%) used darkfield microscopy (DFM). We did not find differences in sensitivity and specificity between the two Tp-PCR methods in the subgroup of studies using adequate reference tests. Among studies using DFM as the reference test, sensitivities were 79.8% (95% confidence intervals [CI], 72.7 to 85.4%) and 71.4% (46.0 to 88.0%) for tpp47-Tp-PCR and polA-Tp-PCR (P = 0.217), respectively; respective specificities were 95.3% (93.5 to 96.6%) and 93.7% (91.8 to 95.2%) (P = 0.304). Our findings suggest that the two Tp-PCR methods have similar accuracy and could be used interchangeably.
机译：现在推荐使用梅毒螺旋体PCR（ Tp -PCR）测试作为诊断原发性或继发性梅毒的有效工具。目的是系统地审查和确定用于 Tp -PCR的最佳特异性靶基因。使用荟萃分析比较了两个主要靶标 tpp47 和 polA 的性能。检索了三个电子书目数据库，它们代表了1990年1月至2015年3月的五个专门针对传染病的会议的摘要书籍。搜索关键词包括（“梅毒”或“梅毒螺旋体”或“神经梅毒”）和（“ PCR”或“ PCR”或“分子扩增”）。我们纳入了评估针对 tpp47 （ tpp 47- Tp -PCR）的 Tp -PCR的性能的诊断研究早期梅毒溃疡中的 polA 基因（ polA-Tp -PCR）。使用QUADAS-2工具根据质量标准对所有研究进行了评估。在确定的37项研究中，有62.2％的人被判断为偏见或适用性低风险。美国疾病控制和预防中心（CDC）最常使用的病例定义是原发性或继发性（早期）梅毒（89.2％; n = 33）; 15（40.5％）个使用了暗场显微镜（DFM）。在使用适当的参考测试的研究亚组中，我们没有发现两种 Tp -PCR方法在敏感性和特异性之间的差异。在使用DFM作为参考测试的研究中， tpp47-Tp -PCR和 polA-Tp -PCR（ P = 0.217）;分别为95.3％（93.5至96.6％）和93.7％（91.8至95.2％）（ P = 0.304）。我们的发现表明，两种 Tp -PCR方法具有相似的准确性，可以互换使用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号