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首页> 外文期刊>Journal of Clinical Microbiology >Multilocus Sequence Analysis of Clinical “Candidatus Neoehrlichia mikurensis” Strains from Europe
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Multilocus Sequence Analysis of Clinical “Candidatus Neoehrlichia mikurensis” Strains from Europe

机译：欧洲临床“ Candidatus Neoehrlichia mikurensis”菌株的多基因座序列分析

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“Candidatus Neoehrlichia mikurensis” is the tick-borne agent of neoehrlichiosis, an infectious disease that primarily affects immunocompromised patients. So far, the genetic variability of “Ca. Neoehrlichia” has been studied only by comparing 16S rRNA genes and groEL operon sequences. We describe the development and use of a multilocus sequence analysis (MLSA) protocol to characterize the genetic diversity of clinical “Ca. Neoehrlichia” strains in Europe and their relatedness to other species within the Anaplasmataceae family. Six genes were selected: ftsZ, clpB, gatB, lipA, groEL, and 16S rRNA. Each MLSA locus was amplified by real-time PCR, and the PCR products were sequenced. Phylogenetic trees of MLSA locus relatedness were constructed from aligned sequences. Blood samples from 12 patients with confirmed “Ca. Neoehrlichia” infection from Sweden (n = 9), the Czech Republic (n = 2), and Germany (n = 1) were analyzed with the MLSA protocol. Three of the Swedish strains exhibited identical lipA sequences, while the lipA sequences of the strains from the other nine patients were identical to each other. One of the Czech strains had one differing nucleotide in the clpB sequence from the sequences of the other 11 strains. All 12 strains had identical sequences for the genes 16S rRNA, ftsZ, gatB, and groEL. According to the MLSA, among the Anaplasmataceae, “Ca. Neoehrlichia” is most closely related to Ehrlichia ruminantium, less so to Anaplasma phagocytophilum, and least to Wolbachia endosymbionts. To conclude, three sequence types of infectious “Ca. Neoehrlichia” were identified: one in the west of Sweden, one in the Czech Republic, and one spread throughout Europe.
机译：“ Candidatus Neoehrlichia mikurensis”是新埃希氏菌病的the传播媒介，它是一种主要影响免疫功能低下患者的传染病。到目前为止，“ Ca ”的遗传变异性。仅通过比较16S rRNA基因和 groEL 操纵子序列来研究“新细菌”。我们描述了开发和使用多基因座序列分析（MLSA）协议来表征临床“ Ca 的遗传多样性。在欧洲的新细菌”菌株及其与Anaplasmataceae科中其他物种的相关性。选择了六个基因： ftsZ ， clpB ， gatB ， lipA ， groEL ，和16S rRNA。通过实时PCR扩增每个MLSA基因座，并对PCR产物进行测序。 MLSA基因座相关性的系统发育树是由比对序列构建的。确诊为“ Ca ”的12名患者的血液样本。分析了瑞典（ n = 9），捷克共和国（ n = 2）和德国（ n = 1）的Neoehrlichia”感染MLSA协议。瑞典的三株菌株表现出相同的 lipA 序列，而其他九名患者的菌株的 lipA 序列彼此相同。捷克菌株中的一个在 clpB 序列中具有一个与其他11个菌株不同的核苷酸。所有12个菌株的16S rRNA， ftsZ ， gatB 和 groEL 基因具有相同的序列。根据MLSA，在无形科中，“ Ca ”。 Neoehrlichia”与反刍动物埃里希氏菌最密切相关，而嗜噬性无浆膜则最不相关，而Wolbachia内共生菌则最不相关。总之，感染性“ Ca ”的三种序列类型。确定了“新狂犬病”：一种在瑞典西部，一种在捷克共和国，另一种遍布整个欧洲。

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