Evaluation of Enzyme Immunoassays and Real-Time PCR for Detecting Shiga Toxin-Producing Escherichia coli in Southern Alberta, Canada

Linda Chui; Laura Patterson-Fortin; Julie Kuo; Vincent Li; Valerie Boras

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Evaluation of Enzyme Immunoassays and Real-Time PCR for Detecting Shiga Toxin-Producing Escherichia coli in Southern Alberta, Canada

机译：在加拿大艾伯塔省南部检测酶免疫测定和实时PCR检测生产志贺毒素的大肠杆菌的评估

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Two immunoassays (Shiga Toxin Chek and Shiga Toxin Quik Chek) and real-time PCR were used to detect Shiga toxin-producing Escherichia coli. For enriched culture, the sensitivity and specificity of the three methods ranged from 80.0% to 98.2% and 98.0% to 100.0%, respectively. STEC isolates were identified in 2.6% of the 784 samples.

机译：两种免疫测定法（志贺毒素Che和志贺毒素快Che）和实时PCR用于检测产志贺毒素的大肠杆菌。对于富集培养，这三种方法的敏感性和特异性分别为80.0％至98.2％和98.0％至100.0％。在784个样本中有2.6％鉴定出STEC分离物。

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《Journal of Clinical Microbiology》 |2015年第3期|共5页
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Linda Chui; Laura Patterson-Fortin; Julie Kuo; Vincent Li; Valerie Boras;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Evaluation of Enzyme Immunoassays and Real-Time PCR for Detecting Shiga Toxin-Producing Escherichia coli in Southern Alberta, Canada [J] . Linda Chui, Laura Patterson-Fortin, Julie Kuo, Journal of Clinical Microbiology . 2015,第3期

机译：在加拿大艾伯塔省南部检测酶免疫测定和实时PCR检测生产志贺毒素的大肠杆菌的评估
2. Prevalence of Shiga Toxin-Producing Escherichia coli as Detected by Enzyme-Linked Immunoassays and Real-Time PCR during the Summer Months in Northern Alberta, Canada [J] . Linda Chui, Mao-Cheng Lee, Kathy Malejczyk, Journal of Clinical Microbiology . 2011,第12期

机译：在加拿大艾伯塔省北部的夏季月份中，通过酶联免疫测定和实时荧光定量PCR检测到产生志贺毒素的大肠杆菌的患病率
3. Comparison between ImmunoCard STAT!? and real-time PCR as screening tools for both O157 : H7 and non-O157 Shiga toxin-producing Escherichia coli in Southern Alberta, Canada [J] . ChuiL., LeeM.-C., AllenR., Diagnostic microbiology and infectious disease . 2013,第1期

机译：ImmunoCard STAT !?之间的比较！和实时PCR分别作为加拿大亚伯达省南部的O157：H7和非O157产志贺毒素的大肠杆菌的筛选工具
4. Two Shiga toxin 2 subtypes in a single Shiga toxin-producing Escherichia coli analyzed by RT-qPCR, MALDI-TOF-TOF-MS and top-down proteomic analysis [C] . Clifton K. Fagerquist, William J. Zaragoza ASMS Conference on Mass Spectrometry and Allied Topics . 2014

机译：通过RT-QPCR，MALDI-TOF-TOF-MS和自上而下的蛋白质组学分析，两种Shiga毒素2种亚型分析的毒素毒素产生的大肠杆菌和自上而下的蛋白质组学分析
5. Development of DNA-tagged liposome biosensing devices and enzyme-linked dot blot assay in combination with nucleic acid sequence-based amplification for rapid detection of viable shiga toxin-producing Escherichia coli. [D] . Kao, Mou-Chieh. 2000

机译：结合DNA标记的脂质体生物传感设备和酶联斑点印迹分析技术的开发，结合基于核酸序列的扩增，可快速检测出产志贺毒素的大肠埃希菌。
6. Evaluation of Enzyme Immunoassays and Real-Time PCR for Detecting Shiga Toxin-Producing Escherichia coli in Southern Alberta Canada [O] . Linda Chui, Laura Patterson-Fortin, Julie Kuo, 2015

机译：在加拿大艾伯塔省南部检测酶免疫法和实时PCR检测生产志贺毒素的大肠杆菌的评估
7. Evaluation of Enzyme Immunoassays and Real-Time PCR for Detecting Shiga Toxin-Producing Escherichia coli in Southern Alberta, Canada [O] . Linda Chui, Laura Patterson-Fortin, Julie Kuo, 2015

机译：酶免疫测定和实时PCR检测南·阿尔伯塔南部南部的滋阴毒素毒素的实时PCR

Evaluation of Enzyme Immunoassays and Real-Time PCR for Detecting Shiga Toxin-Producing Escherichia coli in Southern Alberta, Canada

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