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首页> 外文期刊>Journal of Clinical Microbiology >Automated Broad-Range Molecular Detection of Bacteria in Clinical Samples
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Automated Broad-Range Molecular Detection of Bacteria in Clinical Samples

机译：临床样品中细菌的自动宽范围分子检测

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Molecular detection methods, such as quantitative PCR (qPCR), have found their way into clinical microbiology laboratories for the detection of an array of pathogens. Most routinely used methods, however, are directed at specific species. Thus, anything that is not explicitly searched for will be missed. This greatly limits the flexibility and universal application of these techniques. We investigated the application of a rapid universal bacterial molecular identification method, IS-pro, to routine patient samples received in a clinical microbiology laboratory. IS-pro is a eubacterial technique based on the detection and categorization of 16S-23S rRNA gene interspace regions with lengths that are specific for each microbial species. As this is an open technique, clinicians do not need to decide in advance what to look for. We compared routine culture to IS-pro using 66 samples sent in for routine bacterial diagnostic testing. The samples were obtained from patients with infections in normally sterile sites (without a resident microbiota). The results were identical in 20 (30%) samples, IS-pro detected more bacterial species than culture in 31 (47%) samples, and five of the 10 culture-negative samples were positive with IS-pro. The case histories of the five patients from whom these culture-negative/IS-pro-positive samples were obtained suggest that the IS-pro findings are highly clinically relevant. Our findings indicate that an open molecular approach, such as IS-pro, may have a high added value for clinical practice.
机译：分子检测方法，例如定量PCR（qPCR），已进入临床微生物学实验室，用于检测一系列病原体。但是，最常用的方法是针对特定物种的。因此，任何未明确搜索的内容都会丢失。这极大地限制了这些技术的灵活性和普遍应用。我们调查了快速通用细菌分子鉴定方法IS-pro在临床微生物学实验室接收的常规患者样品中的应用。 IS-pro是一种基于16S-23S rRNA基因间隔区域的检测和分类的真细菌技术，其长度对于每种微生物物种而言都是特定的。由于这是一种开放技术，因此临床医生无需事先决定要寻找的内容。我们使用送入例行细菌诊断测试的66个样品将例行培养与IS-pro进行了比较。样品取自在正常无菌部位（无常生菌群）感染的患者。在20个（30％）样品中结果相同，IS-pro检测到的细菌种类比31个样品（47％）中的培养物多，并且10个培养阴性样品中有5个IS-pro阳性。从获得这些培养阴性/ IS-pro-阳性样本的五名患者的病史表明，IS-pro的发现与临床高度相关。我们的发现表明，开放分子方法（例如IS-pro）可能在临床实践中具有较高的附加值。

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