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首页> 外文期刊>Journal of Clinical Microbiology >Performance of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identifying Clinical Malassezia Isolates
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Performance of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identifying Clinical Malassezia Isolates

机译：基质辅助激光解吸电离的飞行时间质谱技术用于鉴定临床马拉色菌分离物的性能

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The genus Malassezia comprises commensal yeasts on human skin. These yeasts are involved in superficial infections but are also isolated in deeper infections, such as fungemia, particularly in certain at-risk patients, such as neonates or patients with parenteral nutrition catheters. Very little is known about Malassezia epidemiology and virulence. This is due mainly to the difficulty of distinguishing species. Currently, species identification is based on morphological and biochemical characteristics. Only molecular biology techniques identify species with certainty, but they are time-consuming and expensive. The aim of this study was to develop and evaluate a matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) database for identifying Malassezia species by mass spectrometry. Eighty-five Malassezia isolates from patients in three French university hospitals were investigated. Each strain was identified by internal transcribed spacer sequencing. Forty-five strains of the six species Malassezia furfur, M. sympodialis, M. slooffiae, M. globosa, M. restricta, and M. pachydermatis allowed the creation of a MALDI-TOF database. Forty other strains were used to test this database. All strains were identified by our Malassezia database with log scores of >2.0, according to the manufacturer's criteria. Repeatability and reproducibility tests showed a coefficient of variation of the log score values of <10%. In conclusion, our new Malassezia database allows easy, fast, and reliable identification of Malassezia species. Implementation of this database will contribute to a better, more rapid identification of Malassezia species and will be helpful in gaining a better understanding of their epidemiology.
机译：马拉色氏菌属包括人皮肤上的共生酵母。这些酵母菌参与浅表感染，但也分离到深部感染，例如真菌血症，特别是在某些高危患者中，例如新生儿或有肠胃外营养导管的患者。关于马拉色菌的流行病学和毒力知之甚少。这主要是由于难以区分物种。目前，物种鉴定是基于形态和生化特征。只有分子生物学技术才能确定地确定物种，但它们既费时又昂贵。本研究的目的是开发和评估基质辅助激光解吸电离飞行时间（MALDI-TOF）数据库，以通过质谱法鉴定马拉色菌属。对法国三所大学医院的患者中的八十五株马拉色菌进行了调查。通过内部转录间隔子测序鉴定每种菌株。六个种类的马拉色麦草（Malassezia furfur ），交配分枝杆菌（M. sympodialis），食肉分枝杆菌（M. slooffiae），球状分枝杆菌（M. globosa），分枝杆菌（M.stricta）和厚皮分枝杆菌（M. pachydermatis）的45个菌株允许创建MALDI-TOF数据库。使用其他四十株菌株来测试该数据库。根据制造商的标准，所有菌株均由我们的Malassezia数据库鉴定，对数得分大于2.0。重复性和再现性测试显示，对数得分值的变异系数小于10％。总之，我们新的Malassezia数据库可轻松，快速且可靠地识别Malassezia物种。该数据库的实施将有助于更好，更快速地鉴定马拉色菌种，并将有助于更好地了解其流行病学。

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