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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Role of S-100 Immunostaining in Demonstration of Nerve Changes and Quantification of Dendritic Cells in Leprosy
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Role of S-100 Immunostaining in Demonstration of Nerve Changes and Quantification of Dendritic Cells in Leprosy

机译：S-100免疫染色在麻风神经变化和树突状细胞定量中的作用

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Background: A definitive diagnosis of leprosy is based on a demonstration of either acid-fast bacilli or nerve elements within the granulomas. On routine hematoxylin and eosin stains, the nerve fibers are not easily identifiable. In this study, S-100 immunostain is used to highlight the nerve elements and to demonstrate and compare the nerve changes in spectrum of leprosy including reactions.Aim: To demonstrate the nerve changes in spectrum of leprosy using S-100 immunostaining so as to categorize them for the purpose of early diagnosis and treatment. We also want to demonstrate and quantify the dendritic cells in lepromatous spectrum of leprosy using S-100 immunostain.Materials and Methods: Twenty consecutive skin biopsy specimens from patients with histopathological diagnosis of leprosy in the year 2012 were studied. Of these 20 cases, 13 were Borderline Tuberculoid, 1 was of indeterminate leprosy, 1 Borderline Lepromatous, 2 cases of Lepromatous Lep-rosy , 1 case of Type 1 reac-tion and 2 cases of Type 2 reaction. Stains used were Hematoxylin and Eosin stain for the histopathological diagnosis, Fites stain for Bacillary index and S-100 immunoperoxidase staining for nerve changes. 5 cases of granulomatous dermatosis of skin other than leprosy (5 cases of lupus vulgaris) were included as controls.Results: On Hematoxylin and Eosin staining, the nerve fibers showed vertical orientation in relation to epidermis in Borderline Tuberculoid leprosy. In addition , the nerve fibers showed rounded contour in Tuberculoid leprosy. The entire spectrum of leprosy showed evidence of nerve damage in S-100 immunostaining which was categorized in 4 patterns 1. Absent , 2. Fragmented ,3. Discontinuous and 4. Intact. The majority of Borderline Tuberculoid leprosy cases showed absent pattern of nerve damage. Dendritic cells were also positive for S-100 immunostaining with granular positivity in Borderline Tuberculoid Leprosy cases and membranous positivity in Lepromatous spectrum. Conclusion: Nerve damage is seen across the entire spectrum of leprosy and the early identification of this nerve damage using S-100 immunostaining, helps to differentiate between Lepromatous and Tuberculoid leprosy, especially in the borderline and indeterminate forms.
机译：背景：麻风病的明确诊断是基于肉芽肿中耐酸杆菌或神经元的证实。在常规的苏木精和曙红染色中，不容易识别神经纤维。在这项研究中，使用S-100免疫染色来突出神经元，并展示和比较麻风光谱中神经的变化，包括反应。目的：使用S-100免疫染色来展示麻风光谱中的神经变化，以便进行分类为了早期诊断和治疗的目的。我们还想使用S-100免疫染色剂对麻风的麻风光谱中的树突状细胞进行证实和定量。材料与方法：研究对象是2012年从麻风病的组织病理学诊断中连续抽取的20例皮肤活检标本。在这20例病例中，有13例为交界性结核病，1例为不确定性麻风病，1例为交界性麻风病，2例麻风病性红斑病，1例1型反应和2例2型反应。用于组织病理学诊断的污渍为苏木精和曙红染色，用于细菌指数的Fits染色和用于神经变化的S-100免疫过氧化物酶染色。以麻风病以外的肉芽肿性皮肤病5例（寻常性狼疮5例）为对照组。结果：苏木精和伊红染色显示，交界性结核性麻风病的神经纤维相对于表皮呈垂直方向。此外，结核性麻风的神经纤维呈圆形轮廓。麻风病的整个频谱在S-100免疫染色中显示出神经受损的证据，分为4种模式：1.不存在，2.断裂，3。不连续和4.完好无损。大多数交界性结核麻风病例均表现出无神经损伤模式。树突状细胞也对S-100免疫染色呈阳性，在交界性结核性麻风病病例中呈颗粒阳性，而在麻风病谱中膜性阳性。结论：在麻风病的整个范围内都可以看到神经损伤，使用S-100免疫染色法可以早期识别这种神经损伤，有助于区分麻风病和结核性麻风病，特别是处于边缘和不确定状态。

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