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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Molecular Detection of Human Papillomavirus DNA in Oral Lichen Planus Patients
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Molecular Detection of Human Papillomavirus DNA in Oral Lichen Planus Patients

机译：口腔扁平苔藓患者中人乳头瘤病毒DNA的分子检测

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Oral Lichen Planus (OLP) is a chronic inflammatory disease with cell mediated immunopathologic response. Even though OLP has been studied widely for decades, the aetiology of the disease is yet not hypothesised exactly. Autoimmunity, viruses like Hepatitis C Virus (HCV), Human Papillomavirus (HPV), Human Immunodeficiency Virus (HIV), Herpes Simplex Virus (HSV), Candida albicans and stress have been hypothesised in recent articles. The fact that there is paucity of studies on the aetiopathogenesis of oral lichen planus pivoted the study to analyse the presence of HPV in OLP.Aim: The aim of this study is to detect the presence of HPV DNA in the biopsy samples of OLP and also to determine the role played by the virus in aetiopathogenesis of OLP.Materials and Methods: Biopsy samples comprising 15 Oral Lichen Planus tissues and 15 normal buccal mucosa tissues embedded in paraffin blocks were collected from different dental colleges located in Telangana erstwhile Andhra Pradesh. Parameters like age, sex, site of lesions, type of lesion were also compared. The DNA was extracted from the fixed tissue by using modified protocol and Polymerase Chain Reaction (PCR) was performed by using general primers to amplify HPV L1 gene. On comparison of sequences of 10 patients by using Basic Local Alignment Search Tool (BLAST), the sequences were found similar to HPV-18 L1 gene.Results: Thirteen out of 15 (86.6%) lichen planus cases and none out of 15 controls were HPV 18 positive. Compared between males and females and controls significant relation was observed between sex and OLP too. However, no significant relation was observed based on the site of the lesion and based on type of lesion.Conclusion: The molecular studies confirm presence of HPV-18 in OLP tissues of 13 patients with 8 Single Nucleotide Polymorphism (SNPs) indicating probably new variant of HPV-18 in Indian patients.
机译：口腔扁平苔藓（OLP）是一种慢性炎症性疾病，具有细胞介导的免疫病理反应。尽管对OLP的研究已经进行了数十年，但仍没有确切假设该病的病因。在最近的文章中假设自身免疫，丙型肝炎病毒（HCV），人乳头瘤病毒（HPV），人免疫缺陷病毒（HIV），单纯疱疹病毒（HSV），白色念珠菌和压力等病毒和压力。口腔扁平苔藓的致病机理研究很少，这一事实使该研究得以分析OLP中HPV的存在。目的：本研究的目的是检测HPV活检样本中HPV DNA的存在。 OLP并确定病毒在OLP发病机理中的作用。材料与方法：从位于Telangana的不同牙科学院收集包括15个口腔扁平苔藓组织和15个正常颊黏膜组织包埋在石蜡块中的活检样品以前的安得拉邦。还比较了诸如年龄，性别，病变部位，病变类型等参数。通过修改后的方案从固定组织中提取DNA，并使用通用引物扩增HPV L1基因进行聚合酶链反应（PCR）。通过使用基本局部比对搜索工具（BLAST）对10例患者的序列进行比较，发现该序列与HPV-18 L1基因相似。 15名对照是HPV 18阳性。男性和女性之间以及对照组之间的比较也发现性别与OLP之间存在显着的关系。然而，根据病变部位和病变类型没有发现明显的相关性。结论：分子研究证实了13例8个单核苷酸多态性（SNP）患者的OLP组织中存在HPV-18。可能是印度患者中HPV-18的新变体。

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