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首页> 外文期刊>Journal of bacteriology >Isolation and characterization of four types of plasmids from Bacillus subtilis (natto).
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Isolation and characterization of four types of plasmids from Bacillus subtilis (natto).

机译：枯草芽孢杆菌（纳豆）中四种类型质粒的分离和鉴定。

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摘要

Covalently closed circular deoxyribonucleic acids were found in 10 strains of Bacillus natto. The plasmids could be classified into four types on the basis of the molecular weights as well as the patterns in agarose gel electrophoresis after digestion with restriction endonucleases: (i) plasmids (seven were detected) with a molecular weight of 3.6 X 10(6); (ii) plasmids (two were detected) with a molecular weight of 4.0 X 10(6); (iii) plasmids (eight were detected) with a molecular weight of about 34 X 10(6); and (iv), a plasmid with an approximate molecular weight of 46 X 10(6). Out of the 10 plasmid-carrying strains, 6 (IFO3009, IFO3013, IFO3335, IFO13169, IAM1143, and IAM1207) harbored both type 1 and 3 plasmids; 2 (IAM1114 and IAM1168) harbored both type 2 and 3 plasmids, and IFO3936 and IAM1163 carried type 1 and 4 plasmids, respectively.
机译：在纳豆芽孢杆菌的10个菌株中发现了共价闭合的环状脱氧核糖核酸。根据分子量以及限制性内切核酸酶消化后琼脂糖凝胶电泳的模式，质粒可分为四种类型：（i）分子量为3.6 X 10的质粒（检测到七个）（6） ; （ii）分子量为4.0 X 10（6）的质粒（检测到两个）; （iii）分子量约为34 X 10（6）的质粒（检测到8个）; （iv）一种质粒，其分子量约为46 X 10（6）。在10个质粒携带菌株中，有6个（IFO3009，IFO3013，IFO3335，IFO13169，IAM1143和IAM1207）都带有1型和3型质粒。 2（IAM1114和IAM1168）带有2型和3型质粒，而IFO3936和IAM1163分别带有1型和4型质粒。

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