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首页> 外文期刊>Journal of bacteriology >Cell surface components of Streptococcus sanguis: relationship to aggregation, adherence, and hydrophobicity.
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Cell surface components of Streptococcus sanguis: relationship to aggregation, adherence, and hydrophobicity.

机译：血链球菌的细胞表面成分：与聚集，粘附和疏水性的关系。

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Cell surfaces of aggregation, adherence, and hydrophilic variants of Streptococcus sanguis were compared with cell surfaces of the parent strain with regard to their protein and antigenic constituents. Cell surface molecules were released by digestion with mutanolysin. Extraction with sodium dodecyl sulfate (SDS) urea, lithium diiodosalicylate, and boiling water did not solubilize any material which stained with AgNO3 in an SDS-polyacrylamide gel electrophoresis gel. The parent organism S. sanguis 12, which aggregates in saliva, adheres to saliva-coated hydroxyapatite and is hydrophobic, was found to possess a prominently staining 160,000 molecular weight (MW) protein. This protein was almost completely absent from strain 12na, a hydrophobic nonaggregating variant, and was completely absent from the hydrophilic nonaggregating strain 12L. Trypsinization of strain 12 resulted in the coincident loss of the 160,000-MW protein and the ability to aggregate in saliva. Trypsin treatment reduced but did not eliminate the hydrophobic character of the cells. Boiling destroyed their ability to aggregate, but did not alter their hydrophobicity. Cell wall digests of strain 12 contained a number of proteins which were absent from strains 12na and 12L. Mutanolysin digests of cell walls of the hydrophilic strains contained almost no material that was visible in a silver-stained SDS-polyacrylamide gel electrophoresis gel. Culture supernatants contained a number of proteins which were immunologically cross-reactive with cell surface proteins. The hydrophilic organisms released a number of 60,000- to 90,000-MW proteins not seen in culture supernatants from the parent strain.
机译：将血链球菌的聚集，粘附和亲水变体的细胞表面与亲本菌株的细胞表面的蛋白质和抗原成分进行了比较。细胞表面分子通过用变溶素消化而释放。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳凝胶中，用十二烷基硫酸钠（SDS）尿素，二碘水杨酸锂和沸水萃取不会溶解任何被AgNO3污染的物质。发现在唾液中聚集，粘附到唾液包被的羟基磷灰石上并且疏水的亲本微生物S.sanguis 12具有明显染色的160,000分子量（MW）蛋白。该蛋白在疏水性非聚集变体12na中几乎完全不存在，而在亲水性非聚集性菌株12L中完全不存在。菌株12的胰蛋白酶消化导致160,000 MW蛋白的同时丢失和在唾液中聚集的能力。胰蛋白酶处理减少但没有消除细胞的疏水特性。沸腾破坏了它们的聚集能力，但没有改变其疏水性。菌株12的细胞壁消化物含有许多在菌株12na和12L中不存在的蛋白质。亲水菌株细胞壁的变溶素消化物几乎不含在银染的SDS-聚丙烯酰胺凝胶电泳凝胶中可见的物质。培养物上清液含有许多与细胞表面蛋白质发生免疫交叉反应的蛋白质。亲水性生物释放出许多60,000至90,000 MW蛋白，这些蛋白在亲本菌株的培养上清液中未见。

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