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首页> 外文期刊>Journal of bacteriology >Nucleotide sequence and expression of the gene for the site-specific integration protein from bacteriophage HP1 of Haemophilus influenzae.
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Nucleotide sequence and expression of the gene for the site-specific integration protein from bacteriophage HP1 of Haemophilus influenzae.

机译：流感嗜血杆菌噬菌体HP1的位点特异性整合蛋白的核苷酸序列和基因表达。

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摘要

The nucleotide sequence of the leftmost 2,363 base pairs of the HP1 genome, which includes the attachment site (attP) and the integration region, was determined. This sequence contained an open reading frame encoding a 337-residue polypeptide, which is a member of the integrase family of site-specific recombination proteins as judged by sequence comparison. The open reading frame was located immediately adjacent to the att site and was oriented so that initiation of translation would begin distal to the att site and end in its immediate vicinity. Expression of this DNA segment in Escherichia coli provided extracts which promoted site-specific recombination between plasmids containing cloned HP1 attP and Haemophilus influenzae attB sites. This recombination was directional, since no reaction was observed between plasmids containing attR and attL sites. The reaction was stimulated by the accessory protein integration host factor of E. coli. Evidence was also obtained that the integration host factor influenced the levels of HP1 integrase expression. The deduced amino acid sequence of HP1 integrase has remarkable similarity to that deduced for the integrase of coliphage 186.
机译：确定了HP1基因组最左边的2,363个碱基对的核苷酸序列，其中包括附着位点（attP）和整合区。通过序列比较判断，该序列包含编​​码337残基多肽的开放阅读框，该多肽是位点特异性重组蛋白整合酶家族的成员。开放阅读框位于紧邻att位的位置并且定向成使得翻译的起始将在att位的远端开始并在其紧邻处终止。该DNA片段在大肠杆菌中的表达提供了提取物，其促进了含有克隆的HP1 attP和流感嗜血杆菌attB位点的质粒之间的位点特异性重组。该重组是定向的，因为在包含attR和attL位点的质粒之间未观察到反应。该反应由大肠杆菌的辅助蛋白整合宿主因子刺激。还获得证据表明整合宿主因子影响HP1整合酶表达的水平。 HP1整合酶的推导氨基酸序列与大肠杆菌噬菌体186的推导氨基酸序列具有显着相似性。

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