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首页> 外文期刊>Journal of bacteriology >Expression of the cloned Escherichia coli O9 rfb gene in various mutant strains of Salmonella typhimurium.
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Expression of the cloned Escherichia coli O9 rfb gene in various mutant strains of Salmonella typhimurium.

机译：克隆的大肠杆菌O9 rfb基因在鼠伤寒沙门氏菌的各种突变株中的表达。

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摘要

To investigate the effect of chromosomal mutation on the synthesis of rfe-dependent Escherichia coli O9 lipopolysaccharide (LPS), the cloned E. coli O9 rfb gene was introduced into Salmonella typhimurium strains defective in various genes involved in the synthesis of LPS. When E. coli O9 rfb was introduced into S. typhimurium strains possessing defects in rfb or rfc, they synthesized E. coli O9 LPS on their cell surfaces. The rfe-defective mutant of S. typhimurium synthesized only very small amounts of E. coli O9 LPS after the introduction of E. coli O9 rfb. These results confirmed the widely accepted idea that the biosynthesis of E. coli O9-specific polysaccharide does not require rfc but requires rfe. By using an rfbT mutant of the E. coli O9 rfb gene, the mechanism of transfer of the synthesized E. coli O9-specific polysaccharide from antigen carrier lipid to the R-core of S. typhimurium was investigated. The rfbT mutant of the E. coli O9 rfb gene failed to direct the synthesis of E. coli O9 LPS in the rfc mutant strain of S. typhimurium, in which rfaL and rfbT functions are intact, but directed the synthesis of the precursor. Because the intact E. coli O9 rfb gene directed the synthesis of E. coli O9 LPS in the same strain, it was suggested that the rfaL product of S. typhimurium and rfbT product of E. coli O9 cooperate to synthesize E. coli O9 LPS in S. typhimurium.
机译：为了研究染色体突变对依赖rfe的大肠杆菌O9脂多糖（LPS）合成的影响，将克隆的大肠杆菌O9 rfb基因引入鼠伤寒沙门氏菌菌株中，这些菌株在参与LPS合成的各种基因中均存在缺陷。当将大肠杆菌O9 rfb引入在rfb或rfc中具有缺陷的鼠伤寒沙门氏菌菌株中时，他们在细胞表面合成了大肠杆菌O9 LPS。鼠伤寒沙门氏菌的rfe缺陷型突变体在引入大肠杆菌O9 rfb后仅合成了非常少量的大肠杆菌O9 LPS。这些结果证实了广泛接受的想法，即大肠杆菌O9特异性多糖的生物合成不需要rfc但需要rfe。通过使用大肠杆菌O9 rfb基因的rfbT突变体，研究了合成的大肠杆菌O9特异性多糖从抗原载体脂质转移到鼠伤寒沙门氏菌R核的机理。大肠杆菌O9 rfb基因的rfbT突变体未能指导鼠伤寒沙门氏菌rfc突变株中大肠杆菌O9 LPS的合成，其中rfaL和rfbT功能完好无损，但指导了前体的合成。因为完整的大肠杆菌O9 rfb基因指导了同一菌株中大肠杆菌O9 LPS的合成，所以建议鼠伤寒沙门氏菌的rfaL产物和大肠杆菌O9的rfbT产物协同合成大肠杆菌O9 LPS。在鼠伤寒沙门氏菌中。

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