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首页> 外文期刊>Journal of bacteriology >Bacillus subtilis early sporulation genes kinA, spo0F, and spo0A are transcribed by the RNA polymerase containing sigma H.
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Bacillus subtilis early sporulation genes kinA, spo0F, and spo0A are transcribed by the RNA polymerase containing sigma H.

机译：枯草芽孢杆菌早期孢子形成基因kinA，spo0F和spo0A被包含sigma H的RNA聚合酶转录。

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摘要

The Bacillus subtilis genes kinA (spoIIJ), spo0F, and spo0A encode components of the sporulation signal transduction pathway. Recent work has suggested that these genes are transcribed by a minor form of RNA polymerase, E sigma H (sigma H is the product of spo0H, another early sporulation gene). We directly tested this hypothesis by performing in vitro transcription assays with reconstituted E sigma H and a set of plasmids containing the kinA, spo0F, and spo0A promoter regions. We were able to obtain distinct transcripts of the expected sizes with all three genes by using linearized or supercoiled templates. Furthermore, primer extension experiments indicate that the transcription start sites for the three genes in vitro and in vivo are the same. In addition, we measured steady-state levels of kinA, spo0F, and spo0A mRNAs during growth in sporulation medium; all of them were increased at or near the beginning of the stationary phase.
机译：枯草芽孢杆菌基因kinA（spoIIJ），spo0F和spo0A编码孢子形成信号转导途径的成分。最近的研究表明，这些基因是通过次要形式的RNA聚合酶E sigma H（sigma H是另一个早期孢子形成基因spo0H的产物）转录的。我们通过用重构的E sigma H和一组含有kinA，spo0F和spo0A启动子区域的质粒进行体外转录测定直接检验了这一假设。通过使用线性化或超螺旋模板，我们能够通过所有三个基因获得预期大小的独特转录本。此外，引物延伸实验表明体外和体内三个基因的转录起始位点是相同的。另外，我们在孢子形成培养基中生长过程中测量了kinA，spo0F和spo0A mRNA的稳态水平。所有这些都在固定阶段开始时或接近固定阶段时增加。

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