Sequence and overexpression of the menD gene from Escherichia coli.

J L Popp

首页> 外文期刊>Journal of bacteriology >Sequence and overexpression of the menD gene from Escherichia coli.

【24h】

Sequence and overexpression of the menD gene from Escherichia coli.

机译：来自大肠杆菌的menD基因的序列和过表达。

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The menD gene of Escherichia coli codes for the first enzyme of menaquinone biosynthesis, 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) synthase. DNA sequence analysis of menD shows an open reading frame encoding a 52-kilodalton protein. Possible promoter and ribosome binding sites are present. Insertion of the menD gene into a tac promoter expression vector leads to nearly a 100-fold increase in the level of SHCHC synthase activity upon induction with isopropyl-beta-D-thiogalactoside (IPTG). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of [35S]methionine-labeled proteins shows a 61-kilodalton protein produced upon induction of the menD-containing expression vector. This is the first reported sequence analysis of a men gene and the first significant amplification of any of the menaquinone biosynthetic enzymes.

机译：大肠杆菌的menD基因编码甲基萘醌生物合成的第一种酶2-琥珀酰基-6-羟基-2,4-环己二烯-1-羧酸（SHCHC）合酶。 menD的DNA序列分析显示了一个开放阅读框，编码52个千达尔顿蛋白。存在可能的启动子和核糖体结合位点。将menD基因插入tac启动子表达载体后，经异丙基-β-D-硫代半乳糖苷（IPTG）诱导后，SHCHC合酶活性水平提高了近100倍。 [35S]蛋氨酸标记的蛋白质的十二烷基硫酸钠-聚丙烯酰胺钠凝胶电泳显示，诱导含有menD的表达载体后产生的61千达尔顿蛋白质。这是首次报道的一种men基因序列分析，也是任何一种甲萘醌生物合成酶的首次显着扩增。

著录项

来源
《Journal of bacteriology》 |1989年第8期|共6页
作者
J L Popp;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类微生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Stereoselective production of (+)-trans-chrysanthemic acid by a microbial esterase: cloning, nucleotide sequence, and overexpression of the esterase gene of Arthrobacter globiformis in Escherichia coli. [J] . M Nishizawa, M Shimizu, H Ohkawa, Applied and Environmental Microbiology . 1995,第9期

机译：（+）-反式菊苣酸酯通过微生物酯酶的立体选择性产生：克隆，核苷酸序列和球形节杆菌的酯酶基因在大肠杆菌中的过表达。
2. Consequences of the overexpression of a eukaryotic membrane protein, the human KDEL receptor, in Escherichia coli. [J] . Klepsch MM, Persson JO, de Gier JW Journal of Molecular Biology . 2011,第4期

机译：真核细胞膜蛋白，人KDEL受体，在大肠杆菌中过表达的后果。
3. Consequences of membrane protein overexpression in Escherichia coli. [J] . Wagner S, Baars L, Ytterberg AJ, Molecular & cellular proteomics: MCP . 2007,第9期

机译：膜蛋白在大肠杆菌中过表达的后果。
4. BRIEF COMMUNICATION: Expression of innate immune response genes in mammary epithelia following stimulation with lipopolysaecharide or Escherichia coli. [C] . C. STOCKUM, B.J. HAIGH, H.H.D. MEYER, Conference of the New Zealand Society of Animal Production . 2008

机译：简介：用脂多利亚替代术或大肠杆菌刺激后乳腺上皮内先天免疫应答基因的表达。
5. Heterodimeric deoxyguanosine/deoxyadenosine kinase from Lactobacillus acidophilus R-26: Affinity protein purification, molecular cloning, sequence of the genes, and expression in Escherichia coli. [D] . Ma, Grace Tak-Yi. 1993

机译：嗜酸乳杆菌R-26的异二聚体脱氧鸟苷/脱氧腺苷激酶：亲和蛋白纯化，分子克隆，基因序列和在大肠杆菌中的表达。
6. Sequence and overexpression of the menD gene from Escherichia coli. [O] . J L Popp 1989

机译：来自大肠杆菌的menD基因的序列和过表达。
7. Sequence and overexpression of the menD gene from Escherichia coli. [O] . Popp, J L 1989

机译：来自大肠杆菌的menD基因的序列和过表达。
8. Use of Shigella flexneri ipaC and ipaH Gene Sequences for the General Identification of Shigella spp. and Enteroinvasive Escherichia coli. [R] . Venkatesan, M. M., Buysse, J. M., Kopecko, D. J. 1989

机译：使用福氏志贺菌ipaC和ipaH基因序列进行志贺氏菌属的一般鉴定。和肠道侵袭性大肠杆菌。

Sequence and overexpression of the menD gene from Escherichia coli.

摘要

著录项

相似文献

相关主题

期刊订阅