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首页> 外文期刊>Journal of bacteriology >Just-in-Time Control of Spo0A Synthesis in Bacillus subtilis by Multiple Regulatory Mechanisms
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Just-in-Time Control of Spo0A Synthesis in Bacillus subtilis by Multiple Regulatory Mechanisms

机译：通过多种调控机制及时控制枯草芽孢杆菌中Spo0A的合成

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The response regulator Spo0A governs multiple developmental processes in Bacillus subtilis, including most conspicuously sporulation. Spo0A is activated by phosphorylation via a multicomponent phosphorelay. Previous work has shown that the Spo0A protein is not rate limiting for sporulation. Rather, Spo0A is present at high levels in growing cells, rapidly rising to yet higher levels under sporulation-inducing conditions, suggesting that synthesis of the response regulator is subject to a just-in-time control mechanism. Transcription of spo0A is governed by a promoter switching mechanism, involving a vegetative, σA-recognized promoter, Pv, and a sporulation σH-recognized promoter, Ps, that is under phosphorylated Spo0A (Spo0A～P) control. The spo0A regulatory region also contains four (including one identified in the present work) conserved elements that conform to the consensus binding site for Spo0A～P binding sites. These are herein designated O1, O2, O3, and O4 in reverse order of their proximity to the coding sequence. Here we report that O1 is responsible for repressing Pv during the transition to stationary phase, that O2 is responsible for repressing Ps during growth, that O3 is responsible for activating Ps at the start of sporulation, and that O4 is dispensable for promoter switching. We also report that Spo0A synthesis is subject to a posttranscriptional control mechanism such that translation of mRNAs originating from Pv is impeded due to RNA secondary structure whereas mRNAs originating from Ps are fully competent for protein synthesis. We propose that the opposing actions of O2 and O3 and the enhanced translatability of mRNAs originating from Ps create a highly sensitive, self-reinforcing switch that is responsible for producing a burst of Spo0A synthesis at the start of sporulation.
机译：应答调节剂Spo0A支配枯草芽孢杆菌的多个发育过程，包括最明显的孢子形成。 Spo0A通过多组分磷光体的磷酸化作用被激活。先前的工作表明，Spo0A蛋白对孢子形成没有速率限制。而是，Spo0A高水平存在于生长中的细胞中，在诱导芽孢形成的条件下迅速升高至更高水平，这表明响应调节剂的合成受制于及时控制机制。 spo0A 的转录受启动子转换机制的控制，该机制涉及植物营养素σ A 识别的启动子P v 和孢子形成σ< sup> H 识别的启动子P s ，受磷酸化Spo0A（Spo0A〜P）控制。 spo0A 调控区还包含四个（包括本研究中确定的）保守元件，与Spo0A〜P结合位点的共有结合位点一致。这些在本文中以相反的顺序分别称为O 1 ，O 2 ，O 3 和O 4 编码序列。在这里，我们报告O 1 负责抑制过渡到平稳阶段的P v ，O 2 负责抑制P s 在生长过程中，O 3 在孢子形成开始时负责激活P s ，而O 4 是可用于启动子切换。我们还报告说，Spo0A合成受转录后控制机制的影响，由于RNA二级结构阻碍了源自P v 的mRNA的翻译，而源自P s 的mRNA受到了限制。完全胜任蛋白质合成。我们认为O 2 和O 3 的相反作用以及源自P s 的mRNA的增强的可翻译性可创建高度敏感的自增强开关，负责在孢子形成开始时产生Spo0A合成突发。

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