Interaction of Penicillin-Binding Protein 2 with Soluble Lytic Transglycosylase B1 in Pseudomonas aeruginosa

Blaine A. Legaree; Anthony J. Clarke

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Interaction of Penicillin-Binding Protein 2 with Soluble Lytic Transglycosylase B1 in Pseudomonas aeruginosa

机译：青霉素结合蛋白2与铜绿假单胞菌中可溶性溶解性转糖基糖苷酶B1的相互作用

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Soluble lytic transglycosylase B1 from Pseudomonas aeruginosa was coupled to Sepharose and used to immobilize interaction partners from membrane protein extracts. Penicillin-binding protein 2 (PBP2) was identified as a binding partner, suggesting that the two proteins function together in the biosynthesis of peptidoglycan. By use of an engineered truncated derivative, the N-terminal module of PBP2 was found to confer the binding properties.

机译：铜绿假单胞菌的可溶性溶解转糖基酶B1与琼脂糖偶联，用于固定膜蛋白提取物中的相互作用伴侣。青霉素结合蛋白2（PBP2）被确定为结合伴侣，表明这两种蛋白在肽聚糖的生物合成中共同起作用。通过使用工程化的截短的衍生物，发现PBP2的N末端模块赋予了结合特性。

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《Journal of bacteriology》 |2008年第20期|共5页
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Blaine A. Legaree; Anthony J. Clarke;
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1. Characterization of Soluble and Membrane-Bound Family 3 Lytic Transglycosylases from Pseudomonas aeruginosa [J] . Neil T. Blackburn, Anthony J. Clarke Biochemistry . 2002,第3期

机译：铜绿假单胞菌的可溶性和膜绑定的家庭3裂解转糖基酶的表征。
2. The bacterial septal ring protein RlpA is a lytic transglycosylase that contributes to rod shape and daughter cell separation in Pseudomonas aeruginosa [J] . JorgensonM.A., ChenY., YahashiriA., Molecular Microbiology . 2014,第1期

机译：细菌间隔环蛋白RlpA是一种溶菌性转糖基化酶，有助于铜绿假单胞菌的杆状和子代细胞分离
3. Peptidoglycan lytic activity of the Pseudomonas aeruginosa phage phi KZ gp144 lytic transglycosylase [J] . Paradis-Bleau C, Cloutier I, Lemieux L, FEMS Microbiology Letters . 2007,第2期

机译：铜绿假单胞菌噬菌体phi KZ gp144裂解转糖基酶的肽聚糖裂解活性
4. Structure and function of lytic transglycosylases from Pseudomonas aeruginosa [C] . N. T. Blackburn, A. J. Clarke Carbohydrate Bioengineering Meeting . 2002

机译：Pseudomonas Aeruginosa的裂解运动酶的结构和功能
5. Characterization of family three lytic transglycosylases from Pseudomonas aeruginosa. [D] . Blackburn, Neil Thomas. 2002

机译：铜绿假单胞菌的家族三裂解转糖基酶的表征。
6. Interaction of Penicillin-Binding Protein 2 with Soluble Lytic Transglycosylase B1 in Pseudomonas aeruginosa [O] . Blaine A. Legaree, Anthony J. Clarke 2008

机译：青霉素结合蛋白2与铜绿假单胞菌中可溶性溶血性转糖基酶B1的相互作用
7. Interaction of Penicillin-Binding Protein 2 with Soluble Lytic Transglycosylase B1 in Pseudomonas aeruginosa▿ [O] . Legaree, Blaine A., Clarke, Anthony J. 2008

机译：青霉素结合蛋白2与铜绿假单胞菌中可溶性溶血性转糖基酶B1的相互作用
8. Characterization of the 'Pseudomonas aeruginosa recA' Analog and Its Protein Product: 'rec-102' is a Mutant Allele of the 'P. aeruginosa' PAO 'recA' Gene [R] . Kokjohn, T. A. , Miller, R. V. 1987

机译：'铜绿假单胞菌reca'类似物及其蛋白质产物的表征：'rec-102'是'p'的突变等位基因。铜绿假单胞菌'paO'reca'基因

Interaction of Penicillin-Binding Protein 2 with Soluble Lytic Transglycosylase B1 in Pseudomonas aeruginosa

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