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首页> 外文期刊>American Journal of Analytical Chemistry >Evaluation of Standards and Interfering Compounds in the Determination of Phenolics by Folin-Ciocalteu Assay Method for Effective Bioprocessing of Biomass
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Evaluation of Standards and Interfering Compounds in the Determination of Phenolics by Folin-Ciocalteu Assay Method for Effective Bioprocessing of Biomass

机译：叶蛋白 - Ciocalteu测定法测定酚类测定方法中的标准和干扰化合物的评价生物质有效生物处理方法

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摘要

Folin Ciocalteu (F-C) assay is the most widely used and convenient method to determine the total phenolics content in foods, herbs, and other plant extracts. Different phenolics standards such as gallic acid, ferulic acid, chlorogenic acid, catechol, and vanillic acid have been used for calibration curves in this assay method. Comparison of these standards, in single or combination of two or more, for more accurate determination of phenolics has not been reported so far. This study tested five single phenolics and seven combinations of mixed phenolic standards to evaluate the optimal standards for F-C method. The different standards were tested to calculate the phenolic content in three known test solutions. We also evaluated interference effect of various compounds in phenolics estimation by F-C method that is usually present in the lignocellulosic biomass-derived sugar solution, and in food products along with phenolics. Finally, the optimal standards with five phenolics were used for the determination of phenolics in alkali pretreated biomass extract. The results indicated that gallic acid was the best standard among the single phenolic compounds and five phenolic compounds solution was the best standard among the mixed phenolic solutions. The presence of glucose, HMF, furfural, and vitamin-B12 did not interfere in phenolic determination; whereas ascorbic acid, tyrosine, formic acid, and acetic acid strongly interfered the results. The results also showed that biomass pelleting process did not affect the generation of phenolics in alkali pretreatment extract.
机译：Folin Ciocalteu（F-C）测定是最广泛使用和方便的方法，用于确定食品，草药和其他植物提取物中的总酚类含量。不同的酚醛酸，阿魏酸，绿原酸，儿茶酚和香草酸等不同的酚醛化标准用于该测定方法中的校准曲线。到目前为止，这些标准的单一或组合的单一或组合的比较尚未报告酚类的更准确测定。本研究检测了五种单一酚类和混合酚醛标准的七种组合，以评估F-C方法的最佳标准。测试了不同的标准，以计算三种已知的测试溶液中的酚醛含量。我们还评估了通过通常存在于木质纤维素生物质衍生的糖溶液中的F-C方法的酚类估计中各种化合物的干扰效应，以及食品以及酚类。最后，使用具有五种酚类的最佳标准用于测定碱预处理生物质提取物中的酚类。结果表明，无碱酸是单一酚类化合物中最佳标准，五种酚类化合物溶液是混合酚溶液中最佳标准。葡萄糖，HMF，糠醛和维生素-B12的存在并未干扰酚醛测定;抗坏血酸，酪氨酸，甲酸和乙酸强烈干扰了结果。结果还表明生物质造粒过程不影响碱预处理提取物中酚类的产生。

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