• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genomics >Identification of two novel highly inducible promoters from Bacillus licheniformis by screening transcriptomic data
【24h】

Identification of two novel highly inducible promoters from Bacillus licheniformis by screening transcriptomic data

机译：通过筛选转录组数据，鉴定来自芽孢杆菌的两种新型高诱导促进剂

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacillus licheniformis TCCC11148 is an important industrial strain used to produce alkaline protease. In this study, the transcriptome of B. licheniformis TCCC11148 was analyzed by high throughput RNA sequencing (RNA-Seq) to identify genes that are expressed differentially in the different phases were detected using RNA-Seq. In total, 440 differentially expressed genes between the 12?h and 48?h groups were identified, including 267 up- and 173 downregulated genes. Additionally, 198 differentially expressed genes were identified in the 48?h vs. the 60?h group, including 182 up- and 16 downregulated genes. To screen for novel inducible promoters, an alkaline protease reporter gene was used to test 24 promoters from 66 candidate genes with obviously higher expression levels (RPKM values) than the control group based on the transcriptome data of B. licheniformis in different phases. Gene 707, related to coenzyme transport and metabolism, and gene 1004, related to posttranslational modification were identified as likely having inducible promoters. The expression level of recombinant strains with reporter genes under the control of promoters p707 and p1004 were 8 times higher than that of the control group. This study contributes a method for finding useful inducible promoters for industrial use based on transcriptomic data.
机译：Bacillus licheniformis TCCC11148是用于生产碱性蛋白酶的重要产业菌株。在该研究中，通过高通量RNA测序（RNA-SEQ）分析B.LICheniformis TCCC11148的转录组，以鉴定使用RNA-SEQ检测到差异在不同相中表达的基因。总共12？H和48·H组之间的440个差异表达基因，包括267升和173个下调基因。另外，在48〜H组中鉴定了198个差异表达的基因，包括182个高度和16个下调基因。为了筛选新型诱导型启动子，碱性蛋白酶报告基因用于根据不同阶段B. lisheniformis的转录组数据，从66个候选基因测试来自66个候选基因的24个候选基因，其表达水平明显高（RPKM值）。与辅酶传输和代谢相关的基因707和与后期改性有关的基因1004，其可能具有诱导型启动子。在促进剂P707和P1004的控制下，具有报告基因的重组菌株的表达水平为对照组的8倍。该研究有助于寻找基于转录组数据的工业用途的有用诱导型启动子的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号