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首页> 外文期刊>Food Science & Nutrition >Optimization of cellulase production under solid‐state fermentation by a new mutant strain of Trichoderma reesei
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Optimization of cellulase production under solid‐state fermentation by a new mutant strain of Trichoderma reesei

机译：用Trichoderma Reesei的新突变菌株在固态发酵下优化纤维素酶生产

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Nowadays, the use of agricultural by‐products, as the cheap substrate for the production of value‐added products, is of high interest for the researchers and practitioners. Cellulase is a relatively expensive and a very important industrial enzyme where in this study was produced form rice by‐products under solid‐state fermentation. A new mutant of Trichoderma reesei was used for cellulase production. The effective variables were initially screened by “Plackett and Burman design.” Afterward, the main variables including moisture content, P source, incubation temperature, and incubation time were optimized by “one factor at a time design.” Finally, the resulting variables including 74% for moisture content, 2?g/L for K 2 HPO 4 , 30°C of incubation temperature, and 4?days of incubation time were reported as the ultimate optimal condition for cellulase production.
机译：如今，使用农业副产品，作为生产增值产品的廉价基材，对研究人员和从业者具有高兴趣。纤维素酶是一种相对昂贵的且具有非常重要的工业酶，在该研究中在固态发酵下制备了水稻副产物。 Trichoderma Reesei的新突变体用于纤维素酶生产。有效的变量最初被“Plackett和Burman Design”筛选。之后，通过“时间设计的一个因素”，优化包括水分含量，P源，孵育温度和孵育时间的主要变量。最后，将所得变量包括74％的水分含量，2μl，k 2 HPO 4,30℃，孵育温度的30℃，4？天孵育时间作为纤维素酶生产的最终最佳条件。

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