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首页> 外文期刊>Metabolites >Optimizing Secondary Electrospray Ionization High-Resolution Mass Spectrometry (SESI-HRMS) for the Analysis of Volatile Fatty Acids from Gut Microbiome
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Optimizing Secondary Electrospray Ionization High-Resolution Mass Spectrometry (SESI-HRMS) for the Analysis of Volatile Fatty Acids from Gut Microbiome

机译：优化二次电喷雾电离高分辨率质谱（SESI-HRMS），用于分析肠道微生物菌挥发性脂肪酸

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Gut microbiota plays essential roles in maintaining gut homeostasis. The composition of gut microbes and their metabolites are altered in response to diet and remedial agents such as antibiotics. However, little is known about the effect of antibiotics on the gut microbiota and their volatile metabolites. In this study, we evaluated the impact of a moderate level of ampicillin treatment on volatile fatty acids (VFAs) of gut microbial cultures using an optimized real-time secondary electrospray ionization coupled with high-resolution mass spectrometry (SESI-HRMS). To evaluate the ionization efficiency, different types of electrospray solvents and concentrations of formic acid as an additive (0.01, 0.05, and 0.1%, v / v ) were tested using VFAs standard mixture (C 2 –C 7 ). As a result, the maximum SESI-HRMS signals of all studied m/z values were observed from water with 0.01% formic acid than those from the aqueous methanolic solutions. Optimal temperatures of sample inlet and ion chamber were set at 130 °C and 85 °C, respectively. SESI spray pressure at 0.5 bar generated the maximum intensity than other tested values. The optimized SESI-HRMS was then used for the analysis of VFAs in gut microbial cultures. We detected that the significantly elevated C 4 and C 7 VFAs in the headspace of gut microbial cultures six hours after ampicillin treatment (1 mg/L). In conclusion, our results suggested that the optimized SESI-HRMS method can be suitable for the analysis of VFAs from gut microbes in a rapid, sensitive, and non-invasive manner.
机译：Gut Microbiota在维持肠道稳态方面发挥重要作用。齿龈微生物和它们的代谢物的组成是响应于饮食和补种类如抗生素而改变。然而，关于抗生素对肠道微生物的影响很少，并且易患它们的挥发性代谢物。在该研究中，我们使用优化的实时二次电喷雾电离与高分辨率质谱（Sesi-HRMS）相结合，评估了氨苄青霉素治疗对肠道微生物培养的挥发性脂肪酸（VFA）的影响。为了评估电离效率，使用VFAS标准混合物（C 2 -C 7）测试不同类型的电喷雾溶剂和作为添加剂的甲酸浓度（0.01,0.05和0.1％，V / V）。结果，从用0.01％甲酸的水观察到所有研究的M / Z值的最大SESI-HRMS信号比来自甲醇溶液水溶液的水。样品入口和离子室的最佳温度分别设定在130℃和85℃。 SESI喷射压力为0.5巴，产生比其他测试值的最大强度。然后将优化的Sesi-HRMS用于肠道微生物培养物中的VFA分析。我们检测到氨苄青霉素治疗后六小时内半空段的显着升高的C 4和C 7 VFA（1mg / L）。总之，我们的结果表明，优化的SESI-HRMS方法适用于以快速，敏感和非侵入方式从肠微生物的VFA分析。

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