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首页> 外文期刊>Microorganisms >Identification and Pathogenic Potential of Bacillus cereus Strains Isolated from a Dairy Processing Plant Producing PDO Taleggio Cheese
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Identification and Pathogenic Potential of Bacillus cereus Strains Isolated from a Dairy Processing Plant Producing PDO Taleggio Cheese

机译：芽孢杆菌菌株的鉴定和致病潜力从乳制品加工厂生产PDO Taleggio奶酪

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Low levels of contamination by Bacillus cereus at the cheese farm is essential for reducing any opportunity for growth prior consumption. In this study, B. cereus distribution in a plant producing Protected Designation of Origin Taleggio cheese was investigated and the virulence potential of the isolates was evaluated. Seventy-four samples were collected from Food and Non Food Contact Surfaces (FCS, NFCS), saline curd, and Taleggio. The eleven isolates were identified, typified, and clustered. Strains were tested for the production of hemolysins, hemolysin BL (HBL), phosphatidylcholine-specific phospholipase C (PC-PLC), proteases, and biofilm, and for the presence of chromosomal toxin-encoding genes ( sph , plcA , cytK , entFM , bcet , entS , nheA , nheB, nheC ). B. cereus was detected on NFCS, FCS, and curd, but not in Taleggio. The isolates were grouped into six clusters, and all produced PC-PLC, hemolysins, and proteases, and most of them HBL (66.7%). All the clusters harbored the nheA, sph, plcA , entFM , and cytK genes, and some also nheB (83.3%) , nheC (66.7%), bcet (50.0%), and entS (66.7%). All strains showed biofilm-forming ability. Our data reveal possible contamination of production plants and cheese curd by potentially virulent B. cereus , but bacterial absence in Taleggio highlights the efficacy of a proper management of the production phases in assuring consumer’s protection.
机译：奶酪农场的芽孢杆菌污染污染的污染程度对于减少生长的生长机会至关重要。在本研究中，研究了产生了植物的植物中的植物中的培养物分布，并评估了分离株的毒力电位。从食物和非食品接触表面（FCS，NFC），盐水凝乳和塔塔比奥收集七十四个样品。鉴定了11个分离株，典型的和聚集。测试溶菌素的溶质，溶血素BL（HBL），磷脂酰胆碱特异性磷脂脂酶C（PC-PLC），蛋白酶和生物膜，以及存在染色体毒素编码基因（SPH，PLCA，CYTK，ENTFM， BCET，ENTS，NHEA，NHEB，NHEC）。 B.在NFCS，FCS和CURD上检测到Cereus，但不在Taleggio上检测到。将分离物分为六个簇，所有生产的PC-PLC，溶血蛋白和蛋白酶，其中大部分HBL（66.7％）。所有簇都覆盖了NHEA，SPH，PLCA，ENTFM和CYTK基因，以及一些也是NHEB（83.3％），NHEC（66.7％），BCET（50.0％）和ents（66.7％）。所有菌株都显示出生物膜形成能力。我们的数据通过潜在的毒力B.培养物揭示了可能的生产植物和奶酪凝乳的污染，但塔比奥的细菌缺失突出了适当管理生产阶段的功效，以确保消费者的保护。

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