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首页> 外文期刊>Revista rvore >Trocas gasosas influenciam na morfogênese in vitro de duas cultivares de oliveira (Olea europaea L.)
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Trocas gasosas influenciam na morfogênese in vitro de duas cultivares de oliveira (Olea europaea L.)

机译：气态交换两种品种De Oliveira的体外形态发生（Olea Europaea L.）的影响

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摘要

The objectives of this work were to establish cultivars of Olea europaea L., namely 'Arbequina' and 'Maria da Fé', and to evaluate the influence of gas exchange on in vitro morphogenesis of these cultivars. Under aseptic conditions, single-bud nodal segments were previously decontaminated using disinfestation protocol developed in this work. The treatments followed a completely randomized design, in a 23 factorial scheme: two cultivars and two olive (Olive medium) culture media (OM) (OM + 20 μ M zeatin [1]; and OM + 20 μ M zeatin + 10 μM GA3 [2]); and two types of sealing (rigid lid without orifice and porous membrane) in five repetitions/treatment. The experimental unit consisted of four tubes. The following parameters were assessed: percentage of total contamination; percentage of fungal and bacterial contamination; number of explants with swollen buds; number of axillary buds, and oxidation percentages. At 30 days of cultivation, the total contamination indexes of 15% and 8.8% were recorded for 'Arbequina' and 'Maria da Fé', respectively. For bacterial and fungal contamination percentages, respectively, 'Arbequina' had 33.3% and 66.7%, whereas 'Maria da Fé' presented 71.4% and 28.6%. Regarding the number of responsive cultures showing swollen buds, there was no difference between cultivars when rigid lids without porous membranes were used. However, lids with porous membranes led to statistically superior values for 'Arbequina' as compared to 'Maria da Fé'. For the number of shoots, culture medium 1 was statistically superior to 2. There was no statistical difference for oxidation percentages among treatments. Thus, because of the low level of contamination, it is suggested to use the disinfestation protocol developed in this work. In addition, the use of culture medium 1 and lids with porous membranes did favoured further development of axillary buds into plants.
机译：这项工作的目标是建立Olea Europaea L的品种，即'Arbequina'和'Maria daFé'，并评估气体交换对这些品种的体外形态发生的影响。在无菌条件下，先前使用本工作中制定的消防议定书进行单芽节点段。该处理遵循完全随机的设计，在23个派分方案中：两种品种和两种橄榄（橄榄介质）培养基（OM）（OM +20μMZeatin [1];和OM +20μMZeatin +10μmGa3 [2]）;和两种类型的密封（刚性盖子没有孔和多孔膜）在五重复/治疗中。实验单元包括四个管。评估以下参数：总污染的百分比;真菌和细菌污染的百分比;芽胀的外植体的数量;腋芽数量和氧化百分比。培养30天，为“arbequina”和“Maria daFé”分别记录了15％和8.8％的总污染指数。对于细菌和真菌污染百分比，“arbequina”分别为33.3％和66.7％，而“玛丽亚大f”呈现71.4％和28.6％。关于显示芽的响应培养的数量，当使用没有多孔膜的刚性盖子时，栽培品种之间没有差异。然而，与“玛丽亚DAFÉ”相比，盖子具有多孔膜的静音上优越的值为“arbequina”。对于芽的数量，培养基1统计学优于2.治疗中氧化百分比没有统计学差异。因此，由于污染水平低，建议使用在这项工作中开发的消泡方案。此外，使用培养基1和具有多孔膜的盖子的使用使腋芽进一步发展到植物中。

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