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首页> 外文期刊>Open Journal of Animal Sciences >Isolation and characterization of superior rumen bacteria of cattle (Bos taurus) and potential application in animal feedstuff
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Isolation and characterization of superior rumen bacteria of cattle (Bos taurus) and potential application in animal feedstuff

机译：牛（Bos Taurus）优越瘤胃细菌的分离与表征，潜在应用在动物饲料中

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Rumen of cattle harbors many microorganisms responsible for bioconversion of nutrients into a source of energy for the animals. In recent years many rumen microbes have been isolated and characterized by sequence analysis of 16S ribosomal RNA gene. Some of the microbes have also been recommended as feed additives for improving the overall growth or production of animals. Rumen bacteria which have potential application in animal feed stuffs were isolated and characterized in this experiment. Isolation was carried out from the rumen of cattle (Bos taurus) using techniques of serial dilutions and repeated tubing of the selectively enriched microbial cultures by using the specific media for rumen bacteria. All the isolates were then screened for in vitro gas production and cellulase enzyme activity and four superior isolates were selected and characterized. There were 18.00% to 23.00% increases in gas production on addition of these isolates to the rumen fluid of cattle and there was better cellulase enzyme activity. Two isolates were identified as Butyrivibrio fibrisolvens, one isolate as Streptococcus species and one isolate as Clostridium aminophilum. This indicated that, these isolates are superior and may have potential to be used as microbial feed additive in ruminants if fed in higher quantity.
机译：牛的瘤胃哈勃许多负责营养物的生物转化成动物的能量来源。近年来，许多瘤胃微生物被分离，并通过16S核糖体RNA基因的序列分析表征。还推荐了一些微生物作为饲料添加剂，用于改善动物的整体生长或生产。在该实验中分离出具有潜在应用的瘤胃细菌，并表征在该实验中。利用瘤胃细菌的特定介质，使用连续稀释液和通过选择性富集的微生物培养物的重复管道进行分离，从牛（BOS Taurus）中瘤胃和重复的微生物培养物。然后筛选所有分离株，用于体外气体产生，选择纤维素酶活性，并选择四个优异的分离物并表征。在添加这些分离物到牛的瘤胃液中，气体生产增加了18.00±23.00 ％〜23.00 ％。将两种分离物鉴定为抑制纤维糊糊剂，一种分离为链球菌物种，一种分离为梭菌氨基硅藻。这表明，如果以更高的量喂食，这些分离株可以是优异的并且可能在反刍动物中用作微生物饲料添加剂。

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