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首页> 外文期刊>Thoracic cancer. >Low prevalence of Merkel cell polyomavirus in human epithelial thymic tumors
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Low prevalence of Merkel cell polyomavirus in human epithelial thymic tumors

机译：梅尔克尔细胞多马病毒在人上皮胸腺肿瘤中的患病率低

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The etiology of thymic epithelial tumors is unknown. Murine polyomavirus strain PTA has been shown to induce thymomas in mice. Recently, using diverse molecular techniques, we reported the presence of human polyomavirus 7 (HPyV7) in thymic epithelial tumors. In the present study, we investigated the prevalence of Merkel cell polyomavirus (MCPyV) in thymic epithelial tumors. Thirty-six thymomas were screened for MCPyV by PCR and subsequently tested by DNA and RNA in situ hybridization and immunohistochemistry. Twenty-six thymomas were diagnosed with myasthenia gravis (MG). MCPyV DNA was detected by PCR in 7 (19.4%) of the 36 thymic epithelial tumors and in six of these, the presence of MCPyV was confirmed by fluorescence situ hybridization. Of these, 3 (28.6%) revealed weak MCPyV LT-antigen protein expression. In addition, one of the MCPyV positive thymomas tested positive for MCPyV LT RNA with RNAscope. Of interest, two out of the three thymomas that previously tested positive for MCPyV by immunohistochemistry also tested positive for HPyV7. One of the 11 MG-negative and 2 of the 25 MG-positive were positive for MCPyV. MCPyV DNA and MCPyV protein expression can be detected in human epithelial thymoma; however, to a far lesser extent than HPyV7. Our data strongly indicate that because of its infrequent detection and weak expression, MCPyV is unlikely to play an important role in the etiopathogenesis of human thymomas. ? 2019 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd.
机译：胸腺上皮肿瘤的病因未知。已经显示鼠多马达仑菌株PTA诱导小鼠中的胸腺瘤。最近，使用不同的分子技术，我们报道了在胸腺上皮肿瘤中的人聚瘤7（HPYV7）存在。在本研究中，我们调查了Merkel细胞多瘤（McPyV）在胸腺上皮肿瘤中的患病率。通过PCR筛选36种胸腺瘤，随后由DNA和RNA测试原位杂交和免疫组化。用肌肌肌无力（MG）诊断出二十六种胸腺瘤。通过PCR检测到麦PYVDNA，在36涎胸腺上皮肿瘤中的7（19.4％），其中6种，通过荧光原位杂交证实MCPyV的存在。其中，3（28.6％）揭示了McPyV LT-抗原蛋白表达的弱。此外，麦PYV阳性胸膜瘤中的一种用Rnascope测试麦帕维尔RNA的阳性。感兴趣的是，三种胸腺瘤中的两种胸肉中的三种胸腺，以前通过免疫组化测试阳性，对HPYV7进行了阳性。 11 mg阴性和25毫克阳性中的2个中的一个是McPyV的阳性。可以在人上皮胸腺瘤中检测McPyV DNA和McPyV蛋白表达;但是，到远小于HPYV7的程度。我们的数据强烈表明，由于其罕见的检测和弱表达，麦比维不太可能在人胸腺瘤的病因发生中发挥重要作用。 ？ 2019年的作者。中国肺部肿瘤集团和约翰瓦里和儿子澳大利亚发表的胸癌

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