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首页> 外文期刊>Journal of Natural Science Biology and Medicine >X-gal staining of canine skin tissues: A technique with multiple possible applications
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X-gal staining of canine skin tissues: A technique with multiple possible applications

机译：犬皮肤组织的X-gal染色：一种具有多种可能应用的技术

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Background:Estimation of β-galactosidase (βgal) activity in human cells and tissues indicate its possible use as a marker of senescence.Objectives:This study was done to detect senescence-associated βgal (SA-βgal) activity in canine skin tissue by using its substrate 5-bromo-4-chloro-3-indolyl β-D-galactosidase (X-gal).Materials and Methods:Skin samples were collected through rapid necropsy process. The X-gal staining was done by altering different factors of the staining procedure like pH of the reagents and incubation time. Further, effect of tissue preservation procedure was also evaluated.Results:Typical X-gal staining was detected in old dogs’ skin samples and it was detectable both at pH 6 and pH 7.3. The cells present in the inner lining of the hair follicles and sebaceous glands are the major cells that have high SA-βgal activity. The X-gal staining intensity was more prominent in tissues preserved in liquid nitrogen at -196°C than in -80°C freezer. Prolonged incubation period increased the intensity of staining.Conclusions:This study indicates possibility of X-gal staining in canine tissues and opens an avenue for further in-depth studies that might be useful for different research and clinical studies like determination of dog's approximate age.
机译：背景：人细胞和组织中β-半乳糖苷酶（βGAL）活性的估计表明其可能用作衰老的标志物。目的是通过使用来检测甘氨酸皮肤组织中的衰老相关βGAL（SA-βGAL）活性。其基质5-溴-4-氯-3-吲哚基β-D-半乳糖苷酶（X-GAL）。材料和方法：通过快速尸检过程收集皮肤样品。通过改变试剂的pH值和孵育时间的染色过程的不同因素来完成X-GAL染色。此外，还评估了组织保存程序的影响。结果：在旧狗的皮肤样品中检测到典型的X-gal染色，并且在pH6和pH 7.3中检测到它。存在于毛囊和皮脂腺的内衬中的细胞是具有高SA-βGAL活性的主要细胞。在-196℃保存在-196℃的组织中，X-GAL染色强度比在-80°C的冰箱中更突出。延长的潜伏期增加了染色的强度。结论：本研究表明犬类组织中X-gal染色的可能性，并开启了进一步深入研究的途径，这可能对不同的研究和临床研究有用，例如狗的近似年龄的测定。

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