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首页> 外文期刊>International journal of infectious diseases : >Detection of circulating hepatitis B virus immune escape and polymerase mutants among HBV-positive patients attending Institut Pasteur de Bangui, Central African Republic
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Detection of circulating hepatitis B virus immune escape and polymerase mutants among HBV-positive patients attending Institut Pasteur de Bangui, Central African Republic

机译：中非共和国学院 - 非洲共和国学院近班达的循环乙型肝炎病毒免疫逃逸和聚合酶突变体的检测

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Background Previous studies in the Central African Republic (CAR) have reported the presence of hepatitis B virus (HBV) recombinant genotype E/D and a suspicion of immune escape mutants (IEMs), without further investigation into their impact on prevention and diagnosis. Consequently, this study investigated HBV mutations among hepatitis B surface antigen (HBsAg)-positive patients attending Institut Pasteur de Bangui in the CAR. Methods Sera from a total of 118 HBsAg-positive patients with no previous history of HBV treatment or vaccination at the Institut Pasteur de Bangui, were sampled between 2017 and 2019. Subsequently, the region spanning the surface and polymerase genes of HBV was amplified by PCR and sequenced. HBV sequences were genotyped/subgenotyped by phylogenetic analysis and serotyped based on predicted amino acid residues at positions s122, s127, s140, s159, and s160. They were then analyzed for HBV IEMs and polymerase mutations. Results The region spanning the surface and polymerase genes was successfully amplified and sequenced for 51 samples. Of the HBV sequences, 49 were genotype E and two were genotype A subgenotype A1; these were serotyped as ayw4 and ayw1, respectively. Potential IEMs sY100C, sA128V, and sM133T, and several polymerase mutants were identified. Conclusions This study raises awareness of the need for further studies to be conducted on a large scale to better understand HBV mutations for improved disease control and prevention strategies in the country.
机译：背景技术中非共和国（轿车）的先前研究报告存在乙型肝炎病毒（HBV）重组基因型E / D和免疫逃生突变体（IEM）的怀疑，而无需进一步调查它们对预防和诊断的影响。因此，本研究研究了乙型肝炎表面抗原（HBsAg）的HBV突变 - 在汽车中参加Institut Pasteur de Bangui的阳性患者。方法方法在2017年和2019年，在Institut Pasteur de Bangui中没有出现118个HBsAg阳性患者的血清，在Institut Pasteur de bangui之间进行了取样。随后，通过PCR扩增跨越HBV表面和聚合酶基因的区域和聚合酶基因并测序。 HBV序列是通过系统发育分析和基于预测氨基酸残基的基因分型/亚因子，基于预测的氨基酸残基，S122，S127，S140，S159和S160。然后分析它们的HBV IEM和聚合酶突变。结果跨越表面和聚合酶基因的区域成功扩增并测序51个样品。在HBV序列中，49个是基因型E，两个是基因型A株A1;这些分别是AYW4和AYW1的血清型。鉴定了潜在的IEMS SY100C，SA128V和SM133T和几种聚合酶突变体。结论本研究提高了对进一步研究的需求，以便在大规模上进行进一步进行，以更好地了解HBV突变在该国改善疾病控制和预防策略。

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