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A label-free aptamer-based biosensor for microRNA detection by the RNA-regulated fluorescence of malachite green

机译：基于标记的Aptamer基生物传感器，用于孔雀石绿色的RNA调节荧光的MicroRNA检测

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MicroRNAs (miRNAs) have been considered as promising molecular biomarkers for disease diagnosis, prognosis, as well as drug development. Herein, we wish to report a low background and label-free aptamer-based biosensor for miRNA assay by RNA-regulated fluorescence of malachite green (MG). In this biosensor-based strategy, target miRNA can specifically hybridize with the DNA extension template to form the T7 in vitro transcription system. Then the following transcription amplification produces a large number of MG RNA aptamers (MGAs) which light up the fluorescence of the MG, achieving significant fluorescence enhancement for miRNA quantitative analysis. The aptamer-based biosensor exhibits high sensitivity with a quite low detection limit of 10 amol target miRNA and high specificity to clearly discriminate very similar miRNA family members, even only one base difference. Furthermore, we have demonstrated that the biosensor is practical and reliable for the quantitative detection of miRNA in complex real samples.
机译：MicroRNAS（miRNA）被认为是有前途的分子生物标志物，用于疾病诊断，预后以及药物开发。在此，我们希望通过RNA调节的孔雀石绿（Mg）的RNA调节荧光报告MiRNA测定的低背景和无标记的Aptamer基生物传感器。在基于生物传感器的策略中，靶miRNA可以与DNA延伸模板特异性杂交以形成T7体外转录系统。然后，以下转录扩增产生大量的Mg RNA适体（MGA），其点亮Mg的荧光，实现了MiRNA定量分析的显着荧光增强。基于Aptamer的生物传感器具有高灵敏度，具有10 AMOL靶MiRNA的相当低的检测限，高特异性，以明确区分非常相似的miRNA家族成员，甚至只有一个基本差异。此外，我们已经证明了生物传感器是实用可靠的复杂真实样品中miRNA的定量检测。

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