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首页> 外文期刊>RSC Advances >An A–π–A′ structural ratiometric fluorescent probe based on benzo[e]indolium for bisulfite and its application in sugar samples and living cells
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An A–π–A′ structural ratiometric fluorescent probe based on benzo[e]indolium for bisulfite and its application in sugar samples and living cells

机译：基于Benzo [E]吲哚的A-π-A'结构比例荧光探针及其在糖样品和活细胞中的应用

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摘要

An A–π–A′ structural ratiometric fluorescent probe 1 bearing a benzo[ e ]indolium moiety was developed for the detection of HSO _(3) ~(?) in PBS buffer solution. HSO _(3) ~(?) was expected to react with probe 1 via a Michael addition reaction, causing dramatic changes in its emission spectrum (from 578 nm to 466 nm) and an obvious color change from orange red to blue which could be observed by the naked eye. The reaction could be complete in 30 seconds in the sensing system, and displayed a high selectivity and sensitivity for HSO _(3) ~(?) with a detection limit of 15 nM. The practical value of the probe was confirmed for application to detect the level of HSO _(3) ~(?) in sugar samples with good recovery and in living cells.
机译：携带苯并[E]吲哚部分的A-π-A'结构比例荧光探针1用于检测PBS缓冲溶液中的HSO _（3）〜（α）。 HSO _（3）〜（？）预期通过迈克尔加成反应与探针1反应，导致其发射光谱（从578nm至466nm）中的显着变化，并且从橙红到蓝色的显而易见的颜色变化由肉眼观察。在传感系统中，反应可以在30秒内完成，并对HSO _（3）〜（α）的检测限为15nm的高选择性和灵敏度。证实探针的实际值用于检测糖样品中HSO _（3）〜（α）的水平，具有良好的回收和活细胞。

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