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首页> 外文期刊>RSC Advances >An A-pi-A ' structural ratiometric fluorescent probe based on benzo[e]indolium for bisulfite and its application in sugar samples and living cells
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An A-pi-A ' structural ratiometric fluorescent probe based on benzo[e]indolium for bisulfite and its application in sugar samples and living cells

机译：基于苯并[e]吲哚鎓的亚硫酸氢盐A-pi-A'结构比例荧光探针及其在糖样和活细胞中的应用

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摘要

An A-pi-A' structural ratiometric fluorescent probe 1 bearing a benzo[e]indolium moiety was developed for the detection of HSO3- in PBS buffer solution. HSO3- was expected to react with probe 1 via a Michael addition reaction, causing dramatic changes in its emission spectrum (from 578 nm to 466 nm) and an obvious color change from orange red to blue which could be observed by the naked eye. The reaction could be complete in 30 seconds in the sensing system, and displayed a high selectivity and sensitivity for HSO3- with a detection limit of 15 nM. The practical value of the probe was confirmed for application to detect the level of HSO3- in sugar samples with good recovery and in living cells.
机译：开发了带有苯并[e]吲哚部分的A-pi-A'结构比例荧光探针1，用于检测PBS缓冲溶液中的HSO3-。预计HSO3-将通过迈克尔加成反应与探针1反应，从而导致其发射光谱发生剧烈变化（从578 nm到466 nm），并且可以从肉眼观察到明显的从橙红色到蓝色的颜色变化。该反应可在传感系统中在30秒内完成，并显示出对HSO3-的高选择性和灵敏度，检测限为15 nM。证实了该探针的实用价值，可用于检测回收率良好的糖样品和活细胞中的HSO3-水平。

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