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首页> 外文期刊>Acute and critical care. >Feasibility study of incident dark-field video microscope for measuring microcirculatory variables in the mouse dorsal skinfold chamber model
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Feasibility study of incident dark-field video microscope for measuring microcirculatory variables in the mouse dorsal skinfold chamber model

机译：暗场视频显微镜的可行性研究测量小鼠背部薄片腔室模型中微循环变量的测量

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Background Despite the importance of microcirculation in organ function, monitoring microcirculation is not a routine practice. With developments in microscopic technology, incident dark field (IDF) microscopy (Cytocam) has allowed visualization of the microcirculation. Dorsal skinfold chamber (DSC) mouse model has been used to investigate microcirculation physiology. By employing Cytocam-IDF imaging with DSC model to assess microcirculatory alteration in lipopolysaccharide (LPS)-induced endotoxemia, we attempted to validate availability of Cytocam-IDF imaging of microcirculation. Methods DSC was implanted in eight BALB/c mice for each group; control and sepsis. Both groups were given 72 hours to recover from surgery. The sepsis group had an additional 24-hour period of recovery post-LPS injection (4 mg/kg). Subsequently, a video of the microcirculation was recorded using Cytocam. Data on microcirculatory variables were obtained. Electron microscopy was implemented using lanthanum fixation to detect endothelial glycocalyx degradation. Results The microcirculatory flow index was significantly lower (control, 2.8±0.3; sepsis, 2.1±0.8; P=0.033) and heterogeneity index was considerably higher (control, 0.10±0.15; sepsis, 0.53±0.48; P=0.044) in the sepsis group than in the control group. Electron microscopy revealed glycocalyx demolishment in the sepsis group. Conclusions Cytocam showed reliable ability for observing changes in the microcirculation under septic conditions in the DSC model. The convenience and good imaging quality and the automatic analysis software available for Cytocam-IDF imaging, along with the ability to perform real-time in vivo experiments in the DSC model, are expected to be helpful in future microcirculation investigations.
机译：背景技术尽管微循环在器官功能中，但监测微循环不是常规实践。随着显微技术的发展，入射暗场（IDF）显微镜（Cytocam）允许可视化微循环。背部肤质室（DSC）小鼠模型已被用于研究微循环生理学。通过使用与DSC模型的Cytocam-IDF成像来评估脂多糖（LPS）诱导的内毒素血症的微循环变化，我们试图验证微循环的CytoCam-IDF成像的可用性。方法为每组植入八个BALB / C小鼠中的DSC;对照和败血症。两组均为72小时才能从手术中恢复。 SEPSIS组在LPS注射后额外的24小时恢复（4 mg / kg）。随后，使用Cytocam记录微循环的视频。获得了关于微循环变量的数据。使用镧固定来实施电子显微镜，以检测内皮甘油癌降解。结果微循环流量指数显着降低（对照，2.8±0.3;败血症，2.1±0.8; p = 0.033）和异质性指数相当高（对照，0.10±0.15;败血症，0.53±0.48; p = 0.044）败血症组比对照组。电子显微镜揭示了败血症组中的甘油癌脱髓。结论Cytocam显示出可靠的能力，用于观察DSC模型中的静脉化病症下微循环变化的能力。预计DSC模型中的Cytocam-IDF成像的便利性和可用于Cytocam-IDF成像的自动分析软件，以及在DSC模型中的体内实验中执行实时的能力，将有助于未来的微循环调查。

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