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首页> 外文期刊>Journal of Virology >Molecular cloning and long terminal repeat sequences of intracisternal A-particle genes in Mus caroli.
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Molecular cloning and long terminal repeat sequences of intracisternal A-particle genes in Mus caroli.

机译：MUS Caroli中脑内粒子基因的分子克隆与长末端重复序列。

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摘要

We isolated DNA clones of intracisternal A-particle (IAP) genes from the genome of an Asian wild mouse, Mus caroli. A typical M. caroli IAP gene was 6.5 kilobase pairs in length and had long terminal repeat (LTR) sequences at both ends. The size of the LTR was 345 base pairs in clone L20, and two LTRs at both ends of this clone were linked to directly repeating cellular sequences of 6 base pairs. Each LTR possessed most of the structural features commonly associated with the retrovirus LTR. The restriction map of the M. caroli IAP gene resembled that of Mus musculus, although the M. caroli IAP gene was 0.4 kilobase pairs shorter than the M. musculus IAP gene in two regions. Sequence homology between the M. caroli and M. musculus IAP LTRs was calculated as about 80%, whereas the LTR sequence of the Syrian hamster IAP gene was about 60% homologous to the M. caroli LTR. The reiteration frequency of the M. caroli IAP genes was estimated as 200 to 400 copies per haploid genome, which is at least 10 times the reported value. These results suggest that the IAP genes observed in the genus Mus are present in multiple copies with structures closely resembling the integrated retrovirus gene.
机译：我们从亚洲野生小鼠的基因组中分离出心子群A-粒子（IAP）基因的DNA克隆，Mus Caroli。典型的M. caroli Iap基因的长度为6.5千碱基对，两端具有长的末端重复（LTR）序列。 LTR的尺寸为345碱基对在克隆L20中，并且该克隆两端的两个LTR链接到直接重复6个碱基对的细胞序列。每个LTR都具有与逆转录病毒LTR共同关联的大部分结构特征。 M. Caroli IAP基因的限制图类似于MUS Musculus的M. Caroli Iap基因的比例比在两个地区的M. Musculus Iap基因短的0.4千比西对。 M. Caroli和M. Musculus IAP LTR之间的序列同源性计算为约80％，而叙利亚仓鼠IAP基因的LTR序列约为60％与M. Caroli LTR同源。每个单倍体基因组估计M. Caroli IAP基因的重新频率为200至400份，这是报告值的至少10倍。这些结果表明，在MUS属中观察到的IAP基因存在于多个副本中，具有与综合逆转录病毒基因密切相关的结构。

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