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首页> 外文期刊>Molecular and Cellular Biology >Protein-binding sites within the 5' DNase I-hypersensitive region of the chicken alpha D-globin gene.
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Protein-binding sites within the 5' DNase I-hypersensitive region of the chicken alpha D-globin gene.

机译：鸡αD-珠蛋白基因的5'DNA酶I-三敏区域内的蛋白质结合位点。

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We mapped at high resolution and as a function of development the hypersensitive domain in the 5'-flanking region of the chicken alpha D-globin gene and determined the specific protein-binding sites within the domain. The domain extends from -130 to +80 nucleotides (nt) relative to the cap site. DNase I footprinting within intact embryonic erythrocyte nuclei revealed a strongly protected area from -71 to -52 nt. The same area was weakly protected in adult nuclei. A factor was present in extracts of erythrocyte nuclei from both embryos and adults that protected the sequence AAGATAAGG (-63 to -55 nt) in DNase I footprinting experiments; at higher concentrations of extract, sequences immediately adjacent (-73 to -64 and -53 to -38) were also protected. The same pattern of binding was revealed by gel mobility shift assays. The identical AAGATAAGG sequence is found in the 5'-flanking region of the beta rho gene; it competed for binding of the alpha D-specific factor, suggesting that regulatory elements are shared.
机译：我们以高分辨率映射，作为在鸡αD-珠蛋白基因的5'侧翼区域中发育过敏域的函数，并确定该结构域内的特定蛋白质结合位点。该结构域相对于帽位点从-130〜+80核苷酸（NT）延伸。 DNase I在完整的胚胎红细胞核内的脚印显示出从-71至-52 nt的强烈保护区域。在成人核中，相同的区域弱保护。从胚胎和成人的红细胞核的提取物中存在一个因子，该胚胎和成人在DNA酶I占地面积实验中保护序列AAGATAAGG（-63至-55nt）;在较高浓度的提取物中，还保护紧邻（-73至-64和-53至-38）的序列也受到保护。通过凝胶迁移率移位测定揭示了相同的结合模式。相同的AAGATAAGG序列是在βrhO基因的5'侧翼区域中的;它竞争αD特定因素的结合，表明该监管要素是共享的。

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