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首页> 外文期刊>Environmental Science & Technology >Identification of Viral Pathogen Diversity in Sewage Sludge by Metagenome Analysis
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Identification of Viral Pathogen Diversity in Sewage Sludge by Metagenome Analysis

机译：代谢组学分析鉴定污水污泥中的病毒病原多样性

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摘要

The large diversity of viruses that exist in human populations are potentially excreted into sewage collection systems and concentrated in sewage sludge. In the U.S., the primary fate of processed sewage sludge (class B biosolids) is application to agricultural land as a soil amendment To characterize and understand infectious risks associated with land application, and to describe the diversity of viruses in human populations, shotgun viral metagenomics was applied to 10 sewage sludge samples from 5 wastewater treatment plants throughout the continental U.S, each serving between 100 000 and 1000 000 people. Nearly 330 million DNA sequences were produced and assembled, and annotation resulted in identifying 43 (26 DNA, 17 RNA) different types of human viruses in sewage sludge. Novel insights include the high abundance of newly emerging viruses (e.g., Coronavirus HKU1, Klassevirus and Cosavirus) the strong representation of respiratory viruses, and the relatively minor abundance and occurrence of Enteroviruses. Viral metagenome sequence annotations were reproducible and independent PCR-based identification of selected viruses suggests that viral metagenomes were a conservative estimate of the true viral occurrence and diversity. These results represent the most complete description of human virus diversity in any wastewater sample to date, provide engineers and environmental scientists with critical information on important viral agents and routes of infection from exposure to wastewater and sewage sludge, and represent a significant leap forward in understanding the pathogen content of class B biosolids.
机译：人口中存在的各种各样的病毒可能会排入污水收集系统，并集中在污水污泥中。在美国，处理后的污水污泥（B类生物固体）的主要命运是作为土壤改良剂应用于农业土地，以表征和理解与土地应用相关的传染风险，并描述人口中病毒的多样性，shot弹枪病毒宏基因组学应用于来自美国大陆5个污水处理厂的10个污水污泥样本，每个样本服务10万人至10万人。产生并组装了近3.3亿条DNA序列，通过注释可以识别出污水污泥中的43种不同类型的人类病毒（26种DNA，17种RNA）。新颖的见解包括大量新出现的病毒（例如冠状病毒HKU1，克拉斯病毒和柯萨病毒），呼吸道病毒的代表性强，以及相对较少的肠病毒和发生率。病毒元基因组序列注释是可重现的，并且对选定病毒的基于PCR的独立鉴定表明，病毒元基因组是对真实病毒发生和多样性的保守估计。这些结果代表了迄今为止任何废水样品中人类病毒多样性的最完整描述，为工程师和环境科学家提供了重要的病毒制剂和从暴露于废水和污水污泥的感染途径的关键信息，并且在理解上迈出了重要的一步B类生物固体的病原体含量。

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  • 来源
    《Environmental Science & Technology》 |2013年第4期|1945-1951|共7页
  • 作者

    Kyle Bibby; Jordan Peccia;

  • 作者单位

    Department of Chemical and Environmental Engineering, Yale University, Mason Laboratory, 9 Hillhouse Avenue, P.O. Box 208286, New Haven, Connecticut 06520, United States,Department of Civil and Environmental Engineering, University of Pittsburgh, 709 Benedum Hall, Pittsburgh, Pennsylvania 15261, United States;

    Department of Chemical and Environmental Engineering, Yale University, Mason Laboratory, 9 Hillhouse Avenue, P.O. Box 208286, New Haven, Connecticut 06520, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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