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首页> 外文期刊>Environmental toxicology and chemistry >INTERACTION OF ENVIRONMENTAL XENOBIOTICS WITH A MULTIXENOBIOTIC DEFENSE MECHANISM IN THE BAY MUSSEL MYTILUS GALLOPROVINCIALIS FROM THE COAST OF CALIFORNIA
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INTERACTION OF ENVIRONMENTAL XENOBIOTICS WITH A MULTIXENOBIOTIC DEFENSE MECHANISM IN THE BAY MUSSEL MYTILUS GALLOPROVINCIALIS FROM THE COAST OF CALIFORNIA

机译：加利福尼亚州海岸的Mussel Mytilus galloprovinials湾中环境异物与多异物防御机制的相互作用

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摘要

A multixenobiotic defense mechanism similar to a P-glycoprotein multidrug transporter was characterized in the gills of the mussel Mytilus galloprovincialis from Monterey, California. Using a fluorescent dye assay, we measured multixenobiotic transport activity in individual gill cells and assessed the effects of inhibitors or competitive substrates on activity. Natural products that inhibit the mammalian transporter also affected dye transport in the mussel gill. Four moderately hydrophobic pesticides (dacthal, chlorbenside, sulfallate, and pentachlorophenol) and four highly hydrophobic xenobiotics (p, p′-DDT, p, p′-DDD, p, p′-DDE, and Aroclor 1254) all inhibited the efflux of dye from the gills, indicating their interaction with a multixenobiotic transport mechanism in this organism. The reactivity with these highly hydrophobic compounds seen in M. galloprovincialis differs from related transporters found in other organisms. A protein in the gill cross-reacts with antibodies directed against the mammalian multidrug transport protein. The level of protein can be assessed easily by dot blot procedures and may facilitate quantitation of protein levels in field situations.
机译：来自加利福尼亚州蒙特雷的贻贝Mytilus galloprovincialis g的特征是类似于P-糖蛋白多药转运蛋白的多异生物防御机制。使用荧光染料分析，我们测量了单个g细胞中的多异源转运活性，并评估了抑制剂或竞争性底物对活性的影响。抑制哺乳动物转运蛋白的天然产物也影响贻贝g中的染料转运。四种中等疏水性农药（双杀，氯苯甲醚，硫酸盐和五氯酚）和四种高度疏水性外源生物（p，p'-DDT，p，p'-DDD，p，p'-DDE和Aroclor 1254）均抑制了the中的染料，表明它们与该生物中的异种生物转运机制相互作用。在鸡疫支原体中发现的与这些高度疏水性化合物的反应性不同于在其他生物体中发现的相关转运蛋白。 ill中的蛋白质与针对哺乳动物多药转运蛋白的抗体发生交叉反应。蛋白质的水平可以通过斑点印迹程序轻松评估，并且可以在野外情况下促进蛋白质水平的定量。

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