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首页> 外文期刊>Fisheries Science >Genetic evaluation of interindividual relatedness for broodstock management of the rare species barfin flounder Verasper moseri using microsatellite DNA markers
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Genetic evaluation of interindividual relatedness for broodstock management of the rare species barfin flounder Verasper moseri using microsatellite DNA markers

机译：利用微卫星DNA标记对稀有物种巴鳍比目鱼Verasper moseri亲鱼管理的个体间亲缘关系的遗传评价

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摘要

The barfin flounder has been recently declared to be rare, as the number of wild individuals in Japan has greatly reduced since 1975. The Japanese National Center for Stock Enhancement started the stocking program for this species on 1987. In the present study, microsatellite DNA loci were applied in order to determine the pedigree of the hatchery-produced juveniles for stock enhancement. Additionally, using six polymorphic microsatellite markers, the accuracy to assign dyads to categories of kinship was estimated using the relatedness estimator (R xy ), similarity indexes (individual genetic identity [GI] and proportion of shared alleles [P s ], identification of kin groups using unweighted pair group method with arithmetic mean (UPGMA) clustering based on interindividual genetic distances (1-R xy, 1-P s, and 1-Gl), a likelihood ratio approach, and partition methods to separate individuals into cohorts. The results were compared with the pedigree relationship previously obtained from parentage analysis. Estimation of the kinship relationship between individuals could be better inferred by means of UPGMA dendrogram clustering based on 1-Gl or 1-P s as interindividual genetic distances, or using computer software to perform partitioning of set of individuals.
机译：由于自1975年以来日本的野生个体数量大大减少，最近宣布了比芬比目鱼稀有。1987年，日本国家种群增强中心开始对该物种进行放养计划。在本研究中，微卫星DNA位点为了确定孵化场生产的幼体的谱系，以提高种群。此外，使用六个多态微卫星标记，使用相关性估计量（R xy ），相似性指标（个体遗传同一性[GI]和共享等位基因的比例[P s < / sub>]，使用基于个体间遗传距离（1-R xy ，1-P s 和1-Gl）的算术平均值（UPGMA）聚类的非加权成对组方法识别亲属，似然比方法和划分方法将个体分为队列，并将结果与​​先前通过亲子关系分析获得的血统关系进行比较，通过基于1的UPGMA树状图聚类，可以更好地推断个体之间的亲属关系Gl或1-P s 作为个体间的遗传距离，或使用计算机软件对一组个体进行划分。

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