Determination of the native forms of vitamin B-1 in bovine milk using a fast and simplified UHPLC method

Schmidt Anatol; Pratsch Herbert; Schreiner Maximilian G.; Mayer Helmut K.

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Determination of the native forms of vitamin B-1 in bovine milk using a fast and simplified UHPLC method

机译：使用快速简化的UHPLC方法测定牛乳中维生素B-1的天然形式

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The aim of this study was to develop a high-throughput UHPLC method for the determination vitamin B-1 active compounds; thiamin, thiamin monophosphate and thiamin diphosphate in bovine milk. In order to sustain the native vitamin B1 phosphorus esters, sample preparation is crucial. Various acids as well as commonly used enzymes and their enzyme mixtures were compared. Method accuracy was confirmed using certified reference material as well as comparison with the corresponding CEN method, and was found to be satisfactory. Studied milk samples showed significant amounts of thiamin monophosphate, which can make up to 53.9% of the total vitamin B-1 content in commercial milk, and up to 78% in raw milk. Moreover, a tremendous variation of the total content of vitamin B-1 was observed between single cows, which ranged from 0.24 mg/L up to 0.54 mg/L of total vitamin B-1. (C) 2017 Elsevier Ltd. All rights reserved.

机译：这项研究的目的是开发一种用于测定维生素B-1活性化合物的高通量UHPLC方法。牛乳中的硫胺素，单磷酸硫胺素和二磷酸硫胺素。为了维持天然维生素B1磷酸酯，样品制备至关重要。比较了各种酸以及常用的酶及其酶混合物。使用认证的参考材料以及与相应的CEN方法的比较确认了方法的准确性，结果令人满意。研究的牛奶样品显示出大量的硫胺素一磷酸，可占商业牛奶中维生素B-1总量的53.9％，而在原奶中维生素B-1的含量高达78％。此外，在单头母牛之间观察到维生素B-1总含量的巨大变化，其总维生素B-1的范围从0.24 mg / L到0.54 mg / L。（C）2017 Elsevier Ltd.保留所有权利。

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来源
《Food Chemistry》 |2017年第15期|452-457|共6页
作者
Schmidt Anatol; Pratsch Herbert; Schreiner Maximilian G.; Mayer Helmut K.;
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作者单位

BOKU Univ Nat Resources & Life Sci Vienna, Food Chem Lab, Dept Food Sci & Technol, Muthgasse 11, A-1190 Vienna, Austria;

BOKU Univ Nat Resources & Life Sci Vienna, Food Chem Lab, Dept Food Sci & Technol, Muthgasse 11, A-1190 Vienna, Austria;

BOKU Univ Nat Resources & Life Sci Vienna, Food Chem Lab, Dept Food Sci & Technol, Muthgasse 11, A-1190 Vienna, Austria;

BOKU Univ Nat Resources & Life Sci Vienna, Food Chem Lab, Dept Food Sci & Technol, Muthgasse 11, A-1190 Vienna, Austria;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
原文格式 PDF
正文语种 eng
中图分类
关键词
Thiamin; Thiamin phosphate esters; UHPLC; Milk;

机译：硫胺素;硫胺素磷酸酯;UHPLC;牛奶;

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Determination of the native forms of vitamin B-1 in bovine milk using a fast and simplified UHPLC method

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