• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food microbiology >Test sensitivity of a commercial serine protease digestion kit for the detection of Trichinella spiralis and Trichinella pseudospiralis larvae in pig muscle
【24h】

Test sensitivity of a commercial serine protease digestion kit for the detection of Trichinella spiralis and Trichinella pseudospiralis larvae in pig muscle

机译：商业丝氨酸蛋白酶消化试剂盒对猪肌肉中旋毛虫和假旋毛虫幼虫的检测灵敏度

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The reference method for Trichinella detection at meat inspection is the magnetic stirrer method (MSM) utilising HCl-pepsin for pooled sample digestion. Due to availability and quality issues with pepsin, alternative digestion methods are being offered, such as the Priocheck Trichinella AAD kit (T-AAD), based on serine endopeptidase digestion. In this study the T-AAD kit was compared to the reference method.Minced pork samples were spiked with T. spiralis muscle larvae (ML) with- and without capsule or T. pseudospiralis ML, and analysed with both tests. Test results of individually spiked test samples were analysed by generalised linear modelling.The T-AAD test kit was comparable to the reference method for the qualitative detection of T. spiralis in pigs, but not quantitatively. Overall, 94% of spiked T. spiralis were recovered using MSM against 75.2% when using T-AAD (p 0.0001). Using the MSM 80.0% of spiked T. pseudospiralis were recovered against 20% with the T-AAD (p 0.0001).Based on our experience with the T-AAD kit, we strongly recommend validating the method on site prior to introduction into routine diagnostic laboratories, but this will not alleviate the poor test sensitivity of the T-AAD for the detection of T. pseudospiralis.
机译：肉类检查中旋毛虫检测的参考方法是利用盐酸胃蛋白酶进行合并样品消化的磁力搅拌法（MSM）。由于胃蛋白酶的可用性和质量问题，正在提供其他消化方法，例如基于丝氨酸内肽酶消化的Priocheck Trichinella AAD试剂盒（T-AAD）。在这项研究中，将T-AAD试剂盒与参考方法进行了比较。将切碎的猪肉样品掺入带有或不带有胶囊或假螺旋体ML的螺旋体肌幼虫（ML），并用两种测试进行分析。通过广义线性建模分析单个加标样品的测试结果.T-AAD测试试剂盒与定性检测猪螺旋体的参考方法相当，但不定量。总体而言，使用MSM可以回收94％的尖螺旋藻，而使用T-AAD可以回收75.2％（p <0.0001）。使用MSM，使用T-AAD可回收80.0％的加标假螺旋体，而使用T-AAD可回收20％（p <0.0001）。基于我们在T-AAD试剂盒上的经验，我们强烈建议在引入常规方法之前现场验证该方法诊断实验室，但这不会减轻T-AAD对假螺旋体检测的测试敏感性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号