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首页> 外文期刊>Food microbiology >Evaluation of the U.S. Food and Drug Administration validated molecular method for detection of Cyclospora cayetanensis oocysts on fresh and frozen berries
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Evaluation of the U.S. Food and Drug Administration validated molecular method for detection of Cyclospora cayetanensis oocysts on fresh and frozen berries

机译：美国食品和药物管理局（FDA）验证的分子方法用于检测新鲜和冷冻浆果上的Cyclospora cayetanensis卵囊的评估

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摘要

Outbreaks and sporadic cases of Cyclospora cayetanensis have been linked to consumption of berries. The efficacy of the U.S. Food and Drug Administration (FDA) method for detection of C. cayetanensis was evaluated in fresh berries (blackberries, strawberries, blueberries and mixed berries) and in frozen mixed berries. The protocol included seeding with C. cayetanensis oocysts, produce washing, DNA extraction and a dual TaqMan assay. As few as five oocysts were detected in every type of fresh berry analyzed. All berry samples seeded with 200 oocysts were positive and all unseeded berry samples were negative. No significant differences were observed among any of the berry types analyzed in detection rates, C_T values and estimated oocyst recovery percentages. Mixed berries were seeded and frozen for up to seven weeks. As few as five oocysts were also detected. No significant differences were observed in C. cayetanensis C_T values between fresh and frozen mixed berries at any seeding level. In conclusion, the FDA BAM Chapter 19B method for the detection of Cyclospora was robust, consistent, and showed high sensitivity in all types of berries analyzed. Evaluation of the FDA detection method in berries will provide reliable laboratory support for surveillance programs and for outbreak investigations.
机译：环孢菌的暴发和零星病例与食用浆果有关。在新鲜浆果（黑莓，草莓，蓝莓和混合浆果）和冷冻混合浆果中，评估了美国食品药品监督管理局（FDA）检测C. cayetanensis的功效。该方案包括播种C. cayetanensis卵囊，进行洗涤，DNA提取和双重TaqMan测定。在所分析的每种新鲜浆果中，仅检测到五个卵囊。播种有200个卵囊的所有浆果样品均为阳性，所有未播种的浆果样品均为阴性。在检测到的任何浆果类型，检出率，C_T值和估计的卵囊回收率之间均未观察到明显差异。将混合的浆果播种并冷冻长达7周。还检测到少至五个卵囊。在任何播种水平下，新鲜和冷冻混合浆果之间的C. cayetanensis C_T值均无显着差异。总之，FDA BAM第19B章用于检测环孢菌的方法是可靠，一致的，并且在所分析的所有类型的浆果中均显示出高灵敏度。对浆果中FDA检测方法的评估将为监视程序和暴发调查提供可靠的实验室支持。

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