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首页> 外文期刊>Food microbiology >Transposon mutagenesis in Pseudomonas fluorescens reveals genes involved in blue pigment production and antioxidant protection.
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Transposon mutagenesis in Pseudomonas fluorescens reveals genes involved in blue pigment production and antioxidant protection.

机译：荧光荧光荧光笔中的转蒸原体诱变揭示了涉及蓝色颜料生产和抗氧化剂保护的基因。

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摘要

Pseudomonas fluorescens Ps_77 is a blue-pigmenting strain able to cause food product discoloration, causing relevant economic losses especially in the dairy industry. Unlike non-pigmenting P. fluorescens, blue pigmenting strains previously were shown to carry a genomic region that includes homologs of trpABCDF genes, pointing at a possible role of the tryptophan biosynthetic pathway in production of the pigment. Here, we employ random mutagenesis to first identify the genes involved in blue-pigment production in P. fluorescens Ps_77 and second to investigate the biological function of the blue pigment. Genetic analyses based on the mapping of the random insertions allowed the identification of eight genes involved in pigment production, including the second copy of trpB (trpB_1) gene. Phenotypic characterization of Ps_77 white mutants demonstrated that the blue pigment increases oxidative-stress resistance. Indeed, while Ps_77 was growing at a normal rate in presence of 5 mM of H2O2, white mutants were completely inhibited. The antioxidative protection is not available for non-producing bacteria in co-culture with Ps_77.
机译：假单胞菌荧光荧光素PS_77是一种能够引起食品变色的蓝色色素菌株，造成相关的经济损失，特别是在乳制品行业中。与非颜料P.荧光通荧光件不同，先前的蓝色着色菌株被示出携带包括TRPABCDF基因的同源物的基因组区域，指向色氨酸生物合成途径在颜料的生产中的可能作用。在这里，我们采用随机诱变，首先鉴定P.荧光荧光型PS_77中涉及蓝色颜料生产的基因，并探讨了蓝色颜料的生物学功能。基于随机插入的映射的遗传分析允许鉴定八个基因参与颜料生产，包括TRPB（TRPB_1）基因的第二拷贝。 PS_77白色突变体的表型表征证明了蓝色颜料增加氧化胁迫性。实际上，当PS_77以5mm H 2 O 2存在的正常速率生长时，完全抑制了白色突变体。抗氧化保护不适用于与PS_77共同培养的非生产细菌。

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  • 来源
    《Food microbiology》 |2019年第9期|497-503|共7页
  • 作者

    Andreani Nadia Andrea; Carraro Lisa; Zhang Lihong; Vos Michiel; Cardazzo Barbara;

  • 作者单位

    Univ Padua Dept Comparat Biomed & Food Sci Viale Univ 16 I-35020 Padua Italy|Univ Exeter European Ctr Environm & Human Hlth ESI Bldg Penryn Campus Penryn TR10 9FE England|Univ Lincoln Sch Life Sci Joseph Banks Labs Green Lane Lincoln LN6 7DL England;

    Univ Padua Dept Comparat Biomed & Food Sci Viale Univ 16 I-35020 Padua Italy;

    Univ Exeter European Ctr Environm & Human Hlth ESI Bldg Penryn Campus Penryn TR10 9FE England;

    Univ Exeter European Ctr Environm & Human Hlth ESI Bldg Penryn Campus Penryn TR10 9FE England;

    Univ Padua Dept Comparat Biomed & Food Sci Viale Univ 16 I-35020 Padua Italy;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Pseudomonas fluorescens; Transposon mutagenesis; Blue-pigment biosynthesis; Oxidative stress resistance; Tryptophan metabolism;

    机译：假单胞菌荧光;转座子诱变;蓝色颜料生物合成;氧化胁迫性;色氨酸代谢;

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