• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food microbiology >Interactions between spoilage bacteria in tri-species biofilms developed under simulated meat processing conditions
【24h】

Interactions between spoilage bacteria in tri-species biofilms developed under simulated meat processing conditions

机译：在模拟肉类加工条件下发生的三种物种生物膜中腐败细菌之间的相互作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The formation of biofilms in the food industry is a major issue, as they are a frequent source of contamination of products, which can result in significant economic losses for processors through spoilage of foods or pose serious health concerns for consumers when foodborne pathogens are present. In this study, experiments were carried out using CDC Biofilm Reactors to produce biofilms on two test surfaces (polystyrene and stainless steel coupons) under a regimen for simulated meat processing conditions (SMPC). This entailed a 12 day regimen of daily cycles of filling the reactors with a meat slurry and letting stand for 16 h, followed by draining and refilling with water for an 8 h period in order to mimic a possible scenario of fluctuating periods of nutrient availability and starvation in a meat processing facility. Strains of Pseudomonas fluorescens, Lactobacillus plantarum and Leuconostoc pseudomesenteroides were used for mono and mixed cultures biofilms as they are relevant spoilage bacteria in the meat processing industry. In monoculture, the viable cell densities (CFU/cm(2)) of the two lactic acid bacteria species tested were higher for biofilms grown on polystyrene as compared to those obtained on stainless steel, whereas viable cell numbers in P. fluorescens monoculture were surface-independent. Synergistic interactions were demonstrated during growth of multi-species biofilms. Results from experiments where one of the 3 strains was inoculated 24 h before introduction of the other two strains showed increased levels of L. plantarum within biofilms grown on both test surfaces. The model developed here serves as a baseline to study the interactions between potential spoilage bacteria during biofilm development.
机译：食品工业中生物膜的形成是一个重大问题，因为它们是产品污染的常见来源，这可能通过腐败食物或在存在食物丧失的病原体时对消费者产生严重的健康问题来导致处理器的显着经济损失。在该研究中，使用CDC生物膜反应器进行实验，在模拟肉类加工条件（SMPC）的方案下在两个测试表面（聚苯乙烯和不锈钢杯）上产生生物膜。这需要12天的日常循环方案，用肉浆料填充反应器，然后放置16小时，然后用水排出并重新填充8小时，以模仿可能的营养空间的波动期的可能场景和饥饿在肉类加工厂。假单胞菌荧光菌株，乳酸杆菌菌株和Leuconostoc假瘤器口为单体和混合培养物生物膜，因为它们是肉类加工行业的相关腐败细菌。在单苗中，与在不锈钢上获得的那些相比，在聚苯乙烯上生长的生物膜的可活细胞密度（CFU / cm（2））较高，而在不锈钢上获得的那些，而P.荧光细胞数为单一培养物的活性细胞数为表面-独立的。在多种生物膜的生长期间证明了协同相互作用。实验结果是在引入其他两个菌株之前接种了3个菌株中的一种24小时，显示出在两种测试表面上生长的生物膜内的L.Portararum水平增加。这里开发的模型用作研究生物膜发育过程中潜在腐败细菌之间的相互作用的基线。

著录项

  • 来源
    《Food microbiology》 |2019年第9期|515-522|共8页
  • 作者

    Lapointe Caroline; Deschenes Louise; Ells Timothy C.; Bisaillon Yanick; Savard Tony;

  • 作者单位

    Agr & Agrifood Canada St Hyacinthe Res & Dev Ctr 3600 Casavant W St Hyacinthe PQ Canada;

    Agr & Agrifood Canada St Hyacinthe Res & Dev Ctr 3600 Casavant W St Hyacinthe PQ Canada;

    Agr & Agrifood Canada Kentville Res & Dev Ctr 32 Main St Kentville NS Canada;

    Agr & Agrifood Canada St Hyacinthe Res & Dev Ctr 3600 Casavant W St Hyacinthe PQ Canada;

    Agr & Agrifood Canada St Hyacinthe Res & Dev Ctr 3600 Casavant W St Hyacinthe PQ Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Biofilms; Spoilage microorganisms; Meat processing; Stainless steel; Polystyrene;

    机译：生物膜;腐败的微生物;肉类加工;不锈钢;聚苯乙烯;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号