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Selection of indigenous lactic acid bacteria presenting anti-listerial activity, and their role in reducing the maturation period and assuring the safety of traditional Brazilian cheeses

机译:具有抗李斯特菌活性的本地乳酸菌的选择及其在缩短成熟期和确保传统巴西奶酪安全性中的作用

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Artisanal raw milk cheeses are highly appreciated dairy products in Brazil and ensuring their microbiological safety has been a great need. This study reports the isolation and characterization of lactic acid bacteria (LAB) strains with anti-listerial activity, and their effects onListeria monocytogenesduring refrigerated shelf-life of soft Minas cheese and ripening of semi-hard Minas cheese. LAB strains (n = 891) isolated from Minas artisanal cheeses (n = 244) were assessed for anti-listerial activity by deferred antagonism assay at 37 °C and 7 °C. The treatments comprised the production of soft or semi-hard Minas cheeses using raw or pasteurized milk, and including the addition of selected LAB only [Lactobacillus brevis2-392,Lactobacillus plantarum1-399 and 4Enterococcus faecalis(1-37, 2-49, 2-388 and 1-400)],L. monocytogenesonly, selected LAB co-inoculated withL. monocytogenes, or without any added cultures. At 37 °C, 48.1% of LAB isolates showed anti-listerial capacity and 77.5% maintained activity at 7 °C. Selected LAB strains presented a bacteriostatic effect onL. monocytogenesin soft cheese.L. monocytogeneswas inactivated during the ripening of semi-hard cheeses by the mix of LAB added. Times to attain a 4 log-reduction ofL. monocytogeneswere 15 and 21 days for semi-hard cheeses produced with raw and pasteurized milk, respectively. LAB with anti-listerial activity isolated from artisanal Minas cheeses can comprise an additional barrier to L. monocytogenes growth during the refrigerated storage of soft cheese and help shorten the ripening period of semi-hard cheeses aged at ambient temperature.
机译:手工原味奶酪是巴西备受赞誉的乳制品,因此确保其微生物学安全性是非常必要的。这项研究报告了具有抗李斯特菌活性的乳酸菌(LAB)菌株的分离和表征,以及它们在软Minas奶酪的冷藏保存期限和半硬Minas奶酪的熟化过程中对单核细胞增生李斯特菌的影响。从米纳斯手工奶酪(n = 244)分离的LAB菌株(n = 891)在37°C和7°C下通过延缓拮抗作用评估抗李斯特菌活性。这些处理方法包括使用生乳或巴氏消毒的牛奶生产软或半硬米纳斯奶酪,包括仅添加选定的LAB [短杆菌(Lactobacillus brevis2-392),植物乳杆菌(Lactobacillus plantarum1-399)和4粪肠球菌(Enterococcus faecalis)(1-37、2-49、2 -388和1-400)],L。仅单核细胞基因,选择的LAB与L共接种。单核细胞增生性或无其他培养物。在37°C时,有48.1%的LAB分离物显示出抗李斯特菌的能力,在7°C时具有77.5%的维持活性。选定的LAB菌株对L具有抑菌作用。软干酪中的单核细胞增生症通过添加LAB的混合物,在半硬质奶酪的成熟过程中使单核细胞增生作用失活。 L达到4对数减少的时间。用原奶和巴氏灭菌法生产的半硬质奶酪的单核细胞增生分别为15天和21天。从手工米纳斯奶酪中分离出来的具有抗李斯特活性的乳酸菌可以在冷藏软奶酪期间对单核细胞增生李斯特氏菌的生长构成额外的障碍,并有助于缩短在环境温度下陈化的半硬奶酪的成熟期。

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