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Isolation and characterization of non-O157 Shiga toxin-producing Escherichia coli (STEC) isolated from retail ground beef in Santiago, Chile

机译:从智利圣地亚哥的零售碎牛肉中分离出的非O157产志贺毒素的大肠杆菌(STEC)的分离和表征

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摘要

Shiga toxin-producingEscherichia coli(STEC) is one of the main cause of foodborne disease worldwide, but isolation rates or characteristics of this bacteria from ground beef in Chile are unknown. The present study aimed to isolate and characterize non-O157 STEC from ground beef sold at retail in the city of Santiago, Chile. We analyzed 430 ground beef samples for the presence of STEC, and isolated the microorganism in 10% of samples (43/430). We obtained 56 isolates from the 43 positive samples; 55 of these (98.2%) fermented sorbitol. Most isolates (98.2%; 55/56) showed β-glucoronidase activity, and only six (10.7%; 6/56) were resistant to tellurite. Among the virulence factors studied (stx1,stx2,eae, andhlyA),stx2was the only virulence factor in 41% of the isolates (23/56), whereas 10.7% (6/56) of isolates carried a combination of three virulence factors (stx1 + stx2 + hlyA). None of the isolates carried the geneeae. Finally, isolates were neither serogroups O157 nor “big six”. In conclusion, ground beef sold in Santiago, Chile is contaminated with STEC; however, further studies are required for understanding their virulence potential.
机译:产生志贺毒素的大肠埃希菌(STEC)是全世界食源性疾病的主要原因之一,但是从智利的碎牛肉中分离这种细菌的比率或特征尚不清楚。本研究旨在从智利圣地亚哥的零售商店出售的碎牛肉中分离和鉴定非O157 STEC。我们分析了430个碎牛肉样品中是否存在STEC,并在10%的样品中分离了微生物(43/430)。我们从43个阳性样本中获得了56个分离株。其中55份(98.2%)的发酵山梨醇。大多数分离株(98.2%; 55/56)显示出β-葡萄糖醛酸酶活性,只有六个(10.7%; 6/56)对亚碲酸盐具有抗性。在研究的毒力因子(stx1,stx2,eae和hlyA)中,stx2是41%分离株(23/56)中唯一的毒力因子,而10.7%(6/56)分离株携带三种毒力因子( stx1 + stx2 + hlyA)。分离株均无携带。最后,分离株既不是O157血清型也不是“六大”。总之,在智利圣地亚哥出售的碎牛肉受到STEC的污染;但是,需要进一步研究以了解其毒力潜力。

著录项

  • 来源
    《Food microbiology》 |2018年第10期|55-60|共6页
  • 作者单位

    Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile;

    Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile;

    Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile;

    Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile;

    Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile;

    Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Non-O157; STEC; Stx; Ground beef; big six;

    机译:非O157;STEC;Stx;碎牛肉;六大;

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