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首页> 外文期刊>Food microbiology >Microbiological quality of selected ready-to-eat leaf vegetables, sprouts and non-pasteurized fresh fruit-vegetable juices including the presence of Cronobacter spp.
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Microbiological quality of selected ready-to-eat leaf vegetables, sprouts and non-pasteurized fresh fruit-vegetable juices including the presence of Cronobacter spp.

机译：精选即食蔬菜叶，豆芽和未经巴氏杀菌的新鲜水果蔬菜汁的微生物质量，包括克罗诺杆菌属。

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摘要

Bacteria of the genus Cronobacter are emerging food-borne pathogens. Foods contaminated with Cronobacter spp. may pose a risk to infants or adults with suppressed immunity. This study was aimed at determining the microbiological quality of ready-to-eat (RTE) plant-origin food products available on the Polish market with special emphasis on the prevalence of Cronobacter genus bacteria. Analyses were carried out on 60 samples of commercial RTE type plant-origin food products, including: leaf vegetables (20 samples), sprouts (20 samples) and non-pasteurized vegetable, fruit and fruit-vegetable juices (20 samples). All samples were determined for the total count of aerobic meso-philic bacteria (TAMB) and for the presence of Cronobacter spp. The isolates of Cronobacter spp. were subjected to genetic identification and differentiation by 16S rDNA sequencing, PCR-RFLP analysis and RAPD-PCR and evaluation of antibiotic susceptibility by the disk diffusion assay. The TAMB count in samples of lettuces, sprouts and non-pasteurized fruit, vegetable and fruit-vegetable juices was in the range of 5.6-7.6, 6.7-8.4 and 2.9-7.7 log CFU g~(-1), respectively. The presence of Cronobacter spp. was detected in 21 (35%) samples of the products, including in 6 (30%) samples of leaf vegetables (rucola, lamb's lettuce, endive escarola and leaf vegetables mix) and in 15 (75%) samples of sprouts (alfalfa, broccoli, small radish, lentil, sunflower, leek and sprout mix). No presence of Cronobacter spp. was detected in the analyzed samples of non-pasteurized fruit, vegetable and fruit-vegetable juices. The 21 strains of Cronobacter spp. isolated from leaf vegetable and sprouts included: 13 strains of C. sakazakii, 4 strains of C. muytjensii, 2 strains of C. turicensis, one strain of C. malonaticus and one strain of C. condimenti. All isolated C. sakazakii, C. muytjensii, C. turicensis and C. malonaticus strains were sensitive to ampicillin, cefepime, chloramphenicol, gentamycin, streptomycin, tetracycline, ciprofloxacin and cotrimoxazol, whereas the C. condimenti isolate showed intermediate resistance to streptomycin and cotrimoxazole.
机译：克罗诺杆菌属细菌是新兴的食源性病原体。被克罗杆菌属细菌污染的食品。可能会对免疫力低下的婴儿或成人造成危险。这项研究旨在确定波兰市场上现成的即食（RTE）植物来源食品的微生物质量，并特别强调克罗诺杆菌属细菌的流行。对60种商用RTE型植物来源食品的样品进行了分析，包括：叶蔬菜（20个样品），芽苗菜（20个样品）和未经巴氏杀菌的蔬菜，水果和水果蔬菜汁（20个样品）。确定所有样品的需氧中性细菌总数（TAMB）和克氏杆菌属的存在。克氏杆菌属的分离株。通过16S rDNA测序，PCR-RFLP分析和RAPD-PCR进行遗传鉴定和分化，并通过圆盘扩散测定法评估抗生素敏感性。生菜，豆芽和未经巴氏杀菌的水果，蔬菜和水果蔬菜汁样品中的TAMB计数分别在5.6-7.6、6.7-8.4和2.9-7.7 log CFU g〜（-1）范围内。克氏杆菌属的存在。在21（35％）的产品样本中检测到，包括6（30％）的蔬菜叶样品（rucola，羔羊的生菜，菊苣escarola和蔬菜叶混合物）和15（75％）的豆芽样品（苜蓿，西兰花，小萝卜，小扁豆，向日葵，韭菜和新芽混合）。不存在cronobacter spp。在未经巴氏杀菌的水果，蔬菜和水果-蔬菜汁的分析样品中检测到了橙红。 Cronobacter spp的21株。从叶菜和豆芽中分离的菌株包括：13株阪崎假丝酵母，4株穆氏假丝酵母，2株土耳假丝酵母，1株丙酸假丝酵母和1株调味品假丝酵母。所有分离的阪崎肠杆菌，多毛弯曲杆菌，土生弯曲杆菌和丙二酸弯曲杆菌菌株对氨苄青霉素，头孢吡肟，氯霉素，庆大霉素，链霉素，四环素，环丙沙星和科曲莫唑都敏感，而康尼丁霉菌和分离株对中间耐药性中等。

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  • 来源
    《Food microbiology》 |2017年第8期|221-230|共10页
  • 作者

    Anna Berthold-Pluta; Monika Garbowska; Ilona Stefańska; Antoni Pluta;

  • 作者单位

    Division of Milk Biotechnology, Department of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159C St, 02-787 Warsaw, Poland;

    Prof. Waclaw Difbrowski Institute of Agricultural and Food Biotechnology, Inter-Department Problem Croup for Dairy Industries, Rakowiecka 36 St, 02-532 Warsaw, Poland;

    Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SCCW, Ciszewskiego 8 St, 02-787 Warsaw, Poland;

    Division of Milk Biotechnology, Department of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159C St, 02-787 Warsaw, Poland;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Cronobacter spp; Vegetables; Fruits; Ready-to-eat foods; Antibiogram;

    机译：克罗诺杆菌属;蔬菜;水果;即食食品;抗菌素;

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