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Reprint of 'Tracking the blue: A MLST approach to characterise the Pseudomonas fluorescens group'

机译：重印“追踪蓝色：表征荧光假单胞菌群体的MLST方法”

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摘要

The Pseudomonas fluorescens group comprises several closely related species that are involved in food contamination and spoilage. Specifically, the interest in P. fluorescens as a spoiler of dairy products increased after the cases of "blue mozzarella" that occurred in Italy in 2010. A Multilocus Sequence Typing (MLST) scheme was developed and applied to characterise 136 isolates (reference strains and food borne isolates) at strain level, to reveal the genetic relationships among them and to disclose any possible genetic clustering of phenotypic markers involved in food spoilage (protease, lipase, lecithinase activities and pigmented or fluorescent molecule production). The production of dark blue diffusible pigment was evaluated on several bacterial culture media and directly on mozzarella cheese. The MLST scheme provided precise genotyping at the strain level, and the population analyses of the concatenated sequences allowed major taxa to be defined. This approach was revealed to be suitable for tracking the strains according to their origin, such as dairy plants or food matrices. The genetic analysis revealed the presence of a connection between the blue pigment production and a specific phylogenetic cluster. The development of the online database specific to the P. fluorescens group will facilitate the application of the scheme and the sharing of the data.
机译：荧光假单胞菌包括与食物污染和腐败有关的几个密切相关的物种。具体而言，自2010年在意大利发生“蓝色马苏里拉干酪”病例以来，对荧光假单胞菌作为乳制品的破坏者的兴趣有所增加。开发了多基因座序列分型（MLST）方案并将其用于表征136个分离株（参考菌株和食源性分离株），以揭示它们之间的遗传关系，并揭示与食物变质有关的表型标记的任何可能的遗传聚类（蛋白酶，脂肪酶，卵磷脂酶活性以及色素或荧光分子的产生）。在几种细菌培养基上并直接在马苏里拉奶酪上评估了深蓝色可扩散颜料的产生。 MLST方案在菌株水平上提供了精确的基因分型，并且对级联序列的总体分析允许定义主要分类单元。据揭示，该方法适合根据菌株的来源追踪菌株，例如奶制品或食品基质。遗传分析表明，蓝色色素的产生与特定的系统发生簇之间存在联系。专门针对萤光假单胞菌小组的在线数据库的开发将促进该方案的应用和数据共享。

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  • 来源
    《Food microbiology》 |2015年第febaptaa期|148-158|共11页
  • 作者

    N.A. Andreani; M.E. Martino; L. Fasolato; L. Carraro; F. Montemurro; R. Mioni; P. Bordin; B. Cardazzo;

  • 作者单位

    Department of Comparative Biomedicine and Food Science (University of Padova), Legnaro 35010, Padova, Italy;

    Department of Comparative Biomedicine and Food Science (University of Padova), Legnaro 35010, Padova, Italy;

    Department of Comparative Biomedicine and Food Science (University of Padova), Legnaro 35010, Padova, Italy,Viale dell'Universita, 16, Legnaro, 350210 Padova, Italy;

    Department of Comparative Biomedicine and Food Science (University of Padova), Legnaro 35010, Padova, Italy;

    Department of Comparative Biomedicine and Food Science (University of Padova), Legnaro 35010, Padova, Italy;

    Istituto Zooprofilattico Sperimentale delle Venezie (IZSve), Legnaro 35020, Padova, Italy;

    Istituto Zooprofilattico Sperimentale delle Venezie (IZSve), Legnaro 35020, Padova, Italy;

    Department of Comparative Biomedicine and Food Science (University of Padova), Legnaro 35010, Padova, Italy;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Pseudomonas fluorescens group; Multilocus Sequence Typing; Food spoilage; Blue mozzarella;

    机译：荧光假单胞菌群;多基因座序列分型;食物变质;蓝芝士;

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