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首页> 外文期刊>Food microbiology >Investigating boundaries of survival, growth and expression of genes associated with stress and virulence of Listeria monocytogenes in response to acid and osmotic stress
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Investigating boundaries of survival, growth and expression of genes associated with stress and virulence of Listeria monocytogenes in response to acid and osmotic stress

机译：研究与单核细胞增生利斯特氏菌的胁迫和毒力相关的基因的存活，生长和表达边界，以应对酸和渗透胁迫

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摘要

The objective of this study was to correlate the relative transcription of Listeria monocytogenes (strains C5, 6179) stress- (gad2, sigB) and virulence- (prfA) associated genes following habituation at twenty-five pH (4.8, 5.0, 5.2, 5.5, 6.4) and NaCl (2, 4, 6, 8, 10% w/v) combinations at 7 ℃, with the survival against subsequent exposure to severe acid stress (pH 2.0 at 37 ℃). Our findings pointed out the inter-strain variation governing growth inhibiting conditions (pH ≤5.0 and NaCl ≥6%), where C_5 was less affected (a reduction of 2.0-3.0 log CFU/mL) than 6179 which was reduced by 4.0-6.0 log CFU/mL at the end of storage. Nevertheless, the higher the habituation at the growth permitting (pH ≤5.5; NaCl ≤A% w/v) or growth inhibiting conditions, the higher the acquired acid resistance or sensitization, respectively. At day 2, gad2 increased relative transcriptional levels are more related to elevated acid resistance, while at day 6 both gad2 transcriptional levels and upregulation ot sigB were correlated to low log reductions and high D_(pH:2.0)-values against severe acid stress. Regarding virulence, the increased transcriptional levels of prfA at day 2 were correlated to adverse pH and NaCl combinations, while prolonged stay in suboptimal conditions as well as exposure to severe acid stress resulted in general activation of the virulence regulator. Such data could definitely contribute in designing safe intervention strategies and additionally integrate - omics aspects in quantitative microbial risk assessment.
机译：这项研究的目的是在25个pH值（4.8、5.0、5.2、5.5）适应后，将单核细胞增生性李斯特菌（菌株C5、6179）应激-（gad2，sigB）和毒力-（prfA）相关基因的相对转录相关。 ，6.4）和NaCl（2、4、6、8、10％w / v）的组合在7℃时具有存活性，可抵抗随后暴露于严重的酸胁迫（37℃的pH 2.0）。我们的研究结果指出了控制生长抑制条件（pH≤5.0和NaCl≥6％）的菌株间变异，其中C_5受到的影响较小（降低了2.0-3.0 log CFU / mL），而6179受到的影响降低了4.0-6.0在储存结束时记录CFU / mL。然而，在允许生长的条件下（pH≤5.5； NaCl≤A％w / v）或生长抑制条件的适应性越高，获得的耐酸性或敏化性分别越高。在第2天，gad2增加的相对转录水平与提高的酸抗性更相关，而在第6天，gad2转录水平和sigB的上调均与低log降低和高D_（pH：2.0）值对抗严重的酸胁迫相关。关于毒力，第2天prfA转录水平的增加与不良的pH和NaCl组合有关，而长时间处于次优条件下以及暴露于严重的酸胁迫则导致了毒力调节剂的普遍激活。这样的数据肯定会有助于设计安全的干预策略，并进一步将组学方法整合到定量微生物风险评估中。

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  • 来源
    《Food microbiology》 |2015年第febaptab期|231-244|共14页
  • 作者

    I.P. Makariti; A. Printezi; A.E. Kapetanakou; N. Zeaki; P.N. Skandamis;

  • 作者单位

    Laboratory of Food Quality Control & Hygiene, Department of Food Science & Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece;

    Laboratory of Food Quality Control & Hygiene, Department of Food Science & Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece;

    Laboratory of Food Quality Control & Hygiene, Department of Food Science & Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece;

    Applied Microbiology, Department of Chemistry, Faculty of Engineering, Lund University, Lund, Sweden;

    Laboratory of Food Quality Control & Hygiene, Department of Food Science & Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Listeria monocytogenes; Inter-strain variation; Habituation; Osmotic-acid stress; Acid resistance; Relative gene transcription;

    机译：李斯特菌;应变间变化;习惯渗透酸胁迫;耐酸性相对基因转录;

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