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Lactose-mediated carbon catabolite repression of putrescine production in dairy Lactococcus lactis is strain dependent

机译:乳糖介导的乳酸乳球菌对腐胺产生的碳分解代谢物的抑制作用与菌株有关

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摘要

Lactococcus lactis is the lactic acid bacterial (LAB) species most widely used as a primary starter in the dairy industry. However, several strains of L. lactis produce the biogenic amine putrescine via the agmatine deiminase (AGDI) pathway. We previously reported the putrescine biosynthesis pathway in L. lactis subsp. cremoris GE2-14 to be regulated by carbon catabolic repression (CCR) via glucose but not lactose (Linares et al., 2013). The present study shows that both these sugars repress putrescine biosynthesis in L. lactis subsp. lactis T3/33, a strain isolated from a Spanish artisanal cheese. Furthermore, we demonstrated that both glucose and lactose repressed the transcriptional activity of the aguBDAC catabolic genes of the AGDI route. Finally, a screening performed in putrescine-producing dairy L. lactis strains determined that putrescine biosynthesis was repressed by lactose in all the L. lactis subsp. lactis strains tested, but in only one L. lactis subsp. cremoris strain. Given the obvious importance of the lactose-repression in cheese putrescine accumulation, it is advisable to consider the diversity of L. lactis in this sense and characterize consequently the starter cultures to select the safest strains.
机译:乳酸乳球菌是乳酸菌(LAB)物种,在乳品工业中被广泛用作主要的发酵剂。然而,乳酸乳球菌的几种菌株通过胍丁胺脱亚氨酶(AGDI)途径产生生物胺腐胺。我们以前报道了乳酸乳杆菌亚种中的腐胺生物合成途径。 cremoris GE2-14可以通过葡萄糖而不是乳糖通过碳分解代谢抑制(CCR)进行调控(Linares等,2013)。本研究表明,这两种糖均能抑制乳酸乳球菌亚种中的腐胺生物合成。乳酸菌T3 / 33,一种从西班牙手工奶酪中分离出的菌株。此外,我们证明葡萄糖和乳糖均抑制AGDI途径的aguBDAC分解代谢基因的转录活性。最后,在产生腐胺的乳乳酸乳球菌菌株中进行的筛选确定,在所有乳酸乳亚种中乳糖都抑制了腐胺的生物合成。乳酸菌菌株,但仅在一个乳酸杆菌亚种中。 creemoris株。鉴于乳糖抑制在干酪腐胺积累中的重要意义,建议在这种意义上考虑乳酸乳球菌的多样性,并据此表征发酵菌以选择最安全的菌株。

著录项

  • 来源
    《Food microbiology》 |2015年第6期|163-170|共8页
  • 作者单位

    Instituto de Productos Lacteos de Asturias, IPLA-CSIC, Paseo Rio Linares s, 33300 Villaviciosa, Spain;

    Instituto de Productos Lacteos de Asturias, IPLA-CSIC, Paseo Rio Linares s, 33300 Villaviciosa, Spain;

    Instituto de Productos Lacteos de Asturias, IPLA-CSIC, Paseo Rio Linares s, 33300 Villaviciosa, Spain;

    Instituto de Productos Lacteos de Asturias, IPLA-CSIC, Paseo Rio Linares s, 33300 Villaviciosa, Spain;

    Instituto de Productos Lacteos de Asturias, IPLA-CSIC, Paseo Rio Linares s, 33300 Villaviciosa, Spain;

    Instituto de Productos Lacteos de Asturias, IPLA-CSIC, Paseo Rio Linares s, 33300 Villaviciosa, Spain;

    Instituto de Productos Lacteos de Asturias, IPLA-CSIC, Paseo Rio Linares s, 33300 Villaviciosa, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Lactococcus lactis; Biogenic amines; Putrescine; AGDI pathway; CCR; Lactose;

    机译:乳酸乳球菌;生物胺腐胺;AGDI途径;CCR;乳糖;

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