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Molecular characterization of antibiotic resistant Salmonella Typhimurium and Salmonella Kentucky isolated from pre- and post-chill whole broilers carcasses

机译:从冷藏前和冷藏后全肉鸡屠体中分离出的抗生素抗性鼠伤寒沙门氏菌和肯塔基州沙门氏菌的分子特征

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摘要

There is conflicting data regarding whether commercial chilling has any effect on persistence of Salmonella serovars, including antibiotic resistant variants, on chicken carcasses. A total of 309 Salmonella Typhimurium and Salmonella Kentucky isolates recovered from pre- and post-chill whole broiler carcasses were characterized for genetic relatedness using Pulsed Field Gel Electrophoresis (PFGE) and for the presence of virulence factors (invA, pagC, spvC) by PCR and for aerobactin and colicin production by bioassays. A subset of these isolates (n = 218) displaying resistance to either sulfisoxazole and/or cef-tiofur [S. Typhimurium (n = 66) and S. Kentucky (n = 152)] were further tested for the presence of associated antibiotic resistance elements (class-Ⅰ integrons and bla_(CMY) genes) by PCR. All 145 ceftiofur resistant S. Kentucky and S. Typhimurium isolates possessed bla_(CMY) genes. Class-Ⅰ integrons were only detected in 6.1% (n = 4/66) of sulfisoxazole resistant S. Typhimurium isolates. The PFGE analysis revealed the presence of genetically diverse populations within the recovered isolates but clusters were generally concordant with serotypes and antimicrobial resistance profiles. At a 100% pattern similarity index, thirty-six percent of the undistinguishable S. Typhimurium and 22% of the undistinguishable S. Kentucky isolates were recovered from the same chilling step. All isolates possessed the invA and pagC genes, but only 1.4%possessed spvC. Irrespective of the chilling step, there was a significant difference (P < 0.05) in the production of aerobactin and colicin between S. Typhimurium and S. Kentucky isolates. Taken together, these results indicate that chilling impacted the recovery of particular Salmonella clonal groups but had no effect on the presence of class-Ⅰ integrons, bla+_(CMY) genes, and tested virulence factors.
机译:关于商业冷冻是否对沙门氏菌血清(包括抗生素抗性变体)对鸡on体的持久性有任何影响的数据存在矛盾。使用脉冲场凝胶电泳(PFGE)对从冷前和后冷的整个肉鸡屠体中回收的309株鼠伤寒沙门氏菌和肯塔基州沙门氏菌分离株进行遗传相关性分析,并通过PCR鉴定毒力因子(invA,pagC,spvC)的存在以及通过生物测定法生产航空细菌素和大肠菌素。这些分离株的一部分(n = 218)显示出对磺胺异恶唑和/或头孢噻呋的抗性[S.通过PCR进一步检测了鼠伤寒(66例)和肯塔基鼠(152例)是否存在相关的抗生素抗性元件(Ⅰ类整合素和bla_(CMY)基因)。耐头孢噻呋的所有145种S.Kentucky和S.typhimurium菌株均具有bla_(CMY)基因。仅在6.1%(n = 4/66)的耐磺异恶唑的鼠伤寒沙门氏菌分离物中检测到Ⅰ类整合素。 PFGE分析显示,在回收的分离物中存在遗传多样性的种群,但簇通常与血清型和抗菌素耐药性相符。在100%模式相似性指数下,从同一冷却步骤中回收了36%的无法区分的鼠伤寒沙门氏菌和22%的无法区分的肯塔基州鼠疫。所有分离株都具有invA和pagC基因,但只有1.4%拥有spvC。不论冷却步骤如何,鼠伤寒沙门氏菌和肯塔基州沙门氏菌分离株的产气杆菌素和大肠菌素均存在显着差异(P <0.05)。综上所述,这些结果表明,低温影响特定沙门氏菌克隆组的恢复,但对Ⅰ类整合素,bla + _(CMY)基因和测试的毒力因子的存在没有影响。

著录项

  • 来源
    《Food microbiology》 |2014年第4期|6-15|共10页
  • 作者单位

    Food Science and Technology Ph.D. Program, Department of Agriculture, Food and Resource Sciences, 2116 Center for Food Science and Technology, University of Maryland Eastern Shore, Princess Anne, MD 21863, USA;

    Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, MD 20708, USA;

    Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, MD 20708, USA;

    Food Science and Technology Ph.D. Program, Department of Agriculture, Food and Resource Sciences, 2116 Center for Food Science and Technology, University of Maryland Eastern Shore, Princess Anne, MD 21863, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Salmonella Typhimurium; Salmonella Kentucky; Pre- and post-chill; Pulsed-field gel electrophoresis; Virulence factors;

    机译:鼠伤寒沙门氏菌;肯塔基沙门氏菌;冷却前和冷却后;脉冲场凝胶电泳;毒力因子;

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