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首页> 外文期刊>Food microbiology >Inactivation of avirulent pgm~+ and Δpgm Yersinia pestis by ultraviolet light (UV-C)
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Inactivation of avirulent pgm~+ and Δpgm Yersinia pestis by ultraviolet light (UV-C)

机译：紫外线（UV-C）灭活无毒pgm〜+和Δpgm鼠疫耶尔森氏菌

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摘要

Yersinia pestis is the causative agent of bubonic plague. Though not considered a foodborne pathogen, Y. pestis can survive, and even grow, in some foods, and the foodborne route of transmission is not without precedent. As such, concerns exist over the possible intentional contamination of foods with this deadly pathogen. Here we report the inactivation of avirulent (pYV-minus) strains of Y. pestis by ultraviolet light (UV-C, 254 nm). Two strains of Y. pestis containing an intact pgm virulence locus (pgm~+) and strains from which the pgm locus was spontaneously deleted (Δpgm) were tested using cells grown in both logarithmic and stationary phase. The D_(10) values for inactivation (the UV-C dose required to inactivate one log of bacterial cells) of Y. pestis on the surface of agar plates ranged from 0.69 to 1.09 mJ/ cm~2. A significant difference was observed between the inactivation of cells of Y. pestis strain Yokohama grown in logarithmic and stationary phases, but no significant difference between growth phase sensitivity to UV-C was observed in Y. pestis strain Kuma. No difference in D_(10) values was observed between pgm~+ and Apgm strains of Yokohama grown to either logarithmic or stationary phase. A measurable difference was observed between the D_(10) of Kuma pgm~+ and Kuma Δpgm grown in logarithmic phase, but this difference was diminished in the Kuma strains grown to stationary phase. Though strain variations exist, the results showing that UV-C can inactivate Y. pestis cells on agar surfaces suggest that UV-C would be effect in inactivating Y. pestis on food surfaces, particularly foods with a smooth surface.
机译：鼠疫耶尔森氏菌是鼠疫的病原体。鼠疫耶尔森菌虽然不被认为是食源性病原体，但它可以在某些食品中生存甚至生长，而食源性传播途径也不是没有先例。因此，人们担心这种致命病原体可能会故意污染食品。在这里，我们报告通过紫外线（UV-C，254 nm）灭活鼠疫耶尔森氏菌的无毒力（pYV-负）菌株。使用在对数期和固定期生长的细胞，测试了两种含有完整pgm毒力基因座的鼠疫耶尔森氏菌菌株（pgm〜+）和自发删除了pgm基因座的菌株（Δpgm）。琼脂平板表面上的鼠疫耶尔森氏菌灭活的D_（10）值（灭活一log细菌细胞所需的UV-C剂量）范围为0.69至1.09 mJ / cm〜2。在对数和固定相中生长的鼠疫耶尔森氏菌横滨菌株的细胞失活之间观察到显着差异，但在鼠疫耶尔森氏菌菌株Kuma中未观察到生长期对UV-C敏感性之间的显着差异。生长到对数或固定相的横滨的pgm〜+和Apgm菌株之间未观察到D_（10）值的差异。在对数期生长的Kuma pgm〜+的D_（10）和KumaΔpgm之间观察到可测量的差异，但在生长到固定相的Kuma菌株中这种差异减小了。尽管存在应变变化，但结果表明UV-C可以灭活琼脂表面上的鼠疫耶尔森氏菌，这表明UV-C可以灭活食品表面（特别是表面光滑的食品）上的鼠疫耶尔森氏菌。

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  • 来源
    《Food microbiology》 |2014年第12期|168-172|共5页
  • 作者

    George C. Paoli; Christopher H. Sommers; O. Joseph Scullen; Chandi Wijey;

  • 作者单位

    Molecular Characterization of Foodborne Pathogens, US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA;

    Food Safety& Intervention Technologies Research Units, US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA;

    Food Safety& Intervention Technologies Research Units, US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA;

    Molecular Characterization of Foodborne Pathogens, US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Yersinia pestis; Ultraviolet light; Food; pgm locus; Irradiation;

    机译：鼠疫耶尔森菌;紫外光线;餐饮;pgm基因座;辐照;

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