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Inactivation of Bacillus spores inoculated in milk by Ultra High Pressure Homogenization

机译:超高压均质法灭活接种牛奶的芽孢杆菌孢子

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摘要

Ultra High-Pressure Homogenization treatments at 300 MPa with inlet temperatures (Ti) of 55, 65, 75 and 85℃ were applied to commercial Ultra High Temperature treated whole milk inoculated with Bacillus cereus, Bacillus licheniformis. Bacillus sporothermodurans, Bacillus coagulans, Geobacillus stear-othermophilus and Bacillus subtilis spores in order to evaluate the inactivation level achieved. Ultra High-Pressure Homogenization conditions at 300 MPa with Ti = 75 and 85℃ were capable of a spore inactivation of ~5 log CFU/mL. Furthermore, under these processing conditions, commercial sterility (evaluated as the complete inactivation of the inoculated spores) was obtained in milk, with the exception of G. stearothermophilus and B. subtilis treated at 300 MPa with 71 = 75℃. The results showed that G. stearothermophilus and B. subtilis have higher resistance to the Ultra High-Pressure Homogenization treatments applied than the other microorganisms inoculated and that a treatment performed at 300 MPa with 71 = 85℃ was necessary to completely inactivate these microorganisms at the spore level inoculated (~1×10~6 CRI/mL). Besides, a change in the resistance of B. licheniformis, B. sporothermodurans, G. stearothermophilus and B. subtilis spores was observed as the inactivation obtained increased remarkably in treatments performed with 71 between 65 and 75℃. This study provides important evidence of the suitability of UHPH technology for the inactivation of spores in high numbers, leading to the possibility of obtaining commercially sterile milk.
机译:将300兆帕,入口温度(Ti)为55、65、75和85℃的300兆帕特高压均质化处理应用于接种蜡样芽孢杆菌,地衣芽孢杆菌的商业超高温处理全脂牛奶。为了评估达到的灭活水平,芽孢芽孢杆菌,凝结芽孢杆菌,嗜热地芽孢杆菌和枯草芽孢杆菌孢子。 Ti = 75和85℃,在300 MPa的超高压均质条件下能使孢子失活达到约5 log CFU / mL。此外,在这些加工条件下,牛奶中获得了商业无菌(评估为接种的孢子完全失活),除了在300 MPa下用71 = 75℃处理的嗜热脂肪芽孢杆菌和枯草芽孢杆菌外,还具有商业无菌性。结果表明,嗜热脂肪芽孢杆菌和枯草芽孢杆菌对所施加的超高压均质处理具有更高的抵抗力,并且在71℃= 85℃,300 MPa的压力下进行处理才能完全灭活这些微生物。接种的孢子水平(〜1×10〜6 CRI / mL)。此外,观察到地衣芽孢杆菌,孢子疏生芽孢杆菌,嗜热脂肪芽孢杆菌和枯草芽孢杆菌孢子的耐药性发生了变化,因为在71至65℃之间进行的处理中获得的失活显着增加。这项研究为UHPH技术适合大量灭活孢子提供了重要证据,从而有可能获得商业上无菌的牛奶。

著录项

  • 来源
    《Food microbiology》 |2014年第12期|204-210|共7页
  • 作者单位

    Centre Especial de Recerca Planta de Tecnologia dels Aliments (CERPTA), XaRTA, TECNIO, MALTA Consolider, Departament de Ciencia Animal i dels Aliments, Universitat Autonoma de Barcelona, 08193, Bellaterra, Barcelona, Spain,Departamento de Ingenieria Quimica, Alimentosy Ambiental, Universidad de las Americas Puebla, Sta. Catarina Martin Cholula, Puebla C.P. 72810, Mexico;

    Centre Especial de Recerca Planta de Tecnologia dels Aliments (CERPTA), XaRTA, TECNIO, MALTA Consolider, Departament de Ciencia Animal i dels Aliments, Universitat Autonoma de Barcelona, 08193, Bellaterra, Barcelona, Spain;

    Centre Especial de Recerca Planta de Tecnologia dels Aliments (CERPTA), XaRTA, TECNIO, MALTA Consolider, Departament de Ciencia Animal i dels Aliments, Universitat Autonoma de Barcelona, 08193, Bellaterra, Barcelona, Spain;

    Centre Especial de Recerca Planta de Tecnologia dels Aliments (CERPTA), XaRTA, TECNIO, MALTA Consolider, Departament de Ciencia Animal i dels Aliments, Universitat Autonoma de Barcelona, 08193, Bellaterra, Barcelona, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Bacillus spore inactivation; Ultra high-pressure homogenization(UHPH); Whole milk; Non-thermal technologies;

    机译:芽孢杆菌灭活;超高压均质(UHPH);全脂牛奶;非热技术;

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