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首页> 外文期刊>Food microbiology >Genotypic and phenotypic characteristics of Cronobacter species, with particular attention to the newly reclassified species Cronobacter helveticus, Cronobacter pulveris, and Cronobacter zurichensis
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Genotypic and phenotypic characteristics of Cronobacter species, with particular attention to the newly reclassified species Cronobacter helveticus, Cronobacter pulveris, and Cronobacter zurichensis

机译：克氏杆菌属的基因型和表型特征，尤其要注意新近重新分类的克氏杆菌，粉状克氏杆菌和苏氏克氏杆菌

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摘要

In 2013, Enterobacter helveticus, Enterobacter pulveris and Enterobacter turicensis, were reclassified as Cronobacter helveticus, Cronobacter pulveris and Cronobacter zurichensis, respectively. Previously these species had been used as negative controls for some Cronobacter detection assays. This study examined cultural, biochemical and molecular Cronobacter detection and identification assays, with emphasis on the new species. Additionally, 32 Cronobacter genomes were examined for the presence of PCR target genes using the BLAST function of the online Cronobacter PubMLST facility. The results of the cultural methods varied and no single medium was able to correctly detect all Cronobacter spp. Since the supporting databases have not been updated to include the Cronobacter genus, Enterobacter sakazakii was returned for four strains of the newly reclassified species with ID32E and none with API 20E. PCR probes targeting rpoB and ompA could not correctly identify the new Cronobacter spp., due to primer specificity or absent target genes. As neonates have been identified as a high-risk group for infection, international standards require the absence of all Cronobacter species in powdered infant formula. However, many conventional detection methods cannot correctly identify the newly recognized species. Conversely, DNA sequence-based methods can adapt to taxonomic revisions and will likely become more common.
机译：2013年，Helveticus肠杆菌，pulveris肠杆菌和turicensis肠杆菌分别重新分类为Cronobacter helveticus，cronobacter pulveris和cronobacter zurichensis。以前，这些物种已被用作某些Cronobacter检测方法的阴性对照。这项研究检查了文化，生化和分子克氏杆菌检测和鉴定方法，重点是新物种。此外，使用在线克氏杆菌PubMLST设施的BLAST功能，检查了32个克氏杆菌基因组的PCR靶基因是否存在。培养方法的结果各不相同，没有一种培养基能够正确检测出所有克罗诺杆菌属。由于支持数据库尚未更新为包括克罗诺杆菌属，因此，阪崎肠杆菌返回了四个新分类的ID32E菌株，而没有API 20E的菌株。由于引物特异性或靶基因缺失，靶向rpoB和ompA的PCR探针无法正确识别新的Cronobacter spp.。由于新生儿已被确定为感染的高危人群，因此国际标准要求在婴儿配方奶粉中不存在所有克罗诺杆菌种。然而，许多常规的检测方法不能正确地识别新识别的物种。相反，基于DNA序列的方法可以适应分类学修订，并且可能会变得更加普遍。

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  • 来源
    《Food microbiology》 |2014年第12期|226-235|共10页
  • 作者

    E.E. Jackson; H. Sonbol; N. Masood; S.J. Forsythe;

  • 作者单位

    Pathogen Research Group, School of Science and Technology, Nottingham Trent University, Gifton Lane, Nottingham, NG11 8NS, UK;

    Pathogen Research Group, School of Science and Technology, Nottingham Trent University, Gifton Lane, Nottingham, NG11 8NS, UK;

    Pathogen Research Group, School of Science and Technology, Nottingham Trent University, Gifton Lane, Nottingham, NG11 8NS, UK;

    Pathogen Research Group, School of Science and Technology, Nottingham Trent University, Gifton Lane, Nottingham, NG11 8NS, UK;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Cronobacter; Detection methods; Identification methods;

    机译：克罗诺杆菌检测方法;鉴定方法;

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