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Antibiofilm effect of plant derived antimicrobials on Listeria monocytogenes

机译:植物源抗菌剂对单核细胞增生李斯特菌的抗生物膜作用

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The present study investigated the efficacy of sub-inhibitory concentrations (SICs, concentrations not inhibiting bacterial growth) and bactericidal concentrations (MBCs) of four, generally recognized as safe (GRAS), plant-derived antimicrobials (PDAs) in inhibiting Listeria monocytogenes (LM) biofilm formation and inactivating mature LM biofilms, at 37,25 and 4 ℃ on polystyrene plates and stainless-steel coupons. In addition, the effect of SICs of PDAs on the expression of LM genes critical for biofilm synthesis was determined by real-time quantitative PCR. The PDAs and their SICs used for inhibition of biofilm were trans-cinnamaldehyde (TC 0.50, 0.75 mM), carvacrol (CR 0.50,0.65 mM), thymol (TY 0.33,0.50 mM), and eugenol (EG 1.8,2.5 mM), whereas the PDA concentrations used for inactivating mature biofilms were 5.0 and 10.0 mM (TC, CR), 3.3 and 5.0 mM (TY), 18.5 and 25.0 mM (EG). All PDAs inhibited biofilm synthesis and inactivated fully formed LM biofilms on both matrices at three temperatures tested (P < 0.05). Realtime quantitative PCR data revealed that all PDAs down-regulated critical LM biofilm-associated genes (P < 0.05). Results suggest that TC, CR, TY, and EG could potentially be used to control LM biofilms in food processing environments, although further studies under commercial settings are necessary.
机译:本研究调查了四个抑制子单核细胞增生李斯特菌(LM)的亚抑制浓度(SICs,不抑制细菌生长的浓度)和杀菌浓度(MBCs)的功效,通常被认为是安全的(GRAS)植物来源的抗菌剂(PDA)。 )在37,25和4℃下,在聚苯乙烯板和不锈钢试样上形成生物膜并失活成熟的LM生物膜。另外,通过实时定量PCR确定了PDA的SIC对生物膜合成至关重要的LM基因表达的影响。用于抑制生物膜的PDA及其SIC为反式肉桂醛(TC 0.50,0.75 mM),香芹酚(CR 0.50,0.65 mM),百里酚(TY 0.33,0.50 mM)和丁子香酚(EG 1.8,2.5 mM),而用于灭活成熟生物膜的PDA浓度为5.0和10.0 mM(TC,CR),3.3和5.0 mM(TY),18.5和25.0 mM(EG)。在三种测试温度下,所有PDA均抑制了两种基质上的生物膜合成并使失活的完全形成的LM生物膜失活(P <0.05)。实时定量PCR数据显示,所有PDA均下调了与LM生物膜相关的关键基因(P <0.05)。结果表明,TC,CR,TY和EG可能可用于控制食品加工环境中的LM生物膜,尽管有必要在商业环境下进行进一步研究。

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